Difference between revisions of "Part:BBa K741009"
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<partinfo>BBa_K741009 short</partinfo> | <partinfo>BBa_K741009 short</partinfo> | ||
− | Here, pCon(J23108) constantly expressing the fusion protein crogfp at 50% of the strength of pCon(J23100). | + | Here, pCon(J23108) constantly expressing the fusion protein crogfp(BBa_K741006) at 50% of the strength of pCon(J23100). |
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Latest revision as of 01:18, 3 October 2012
pCon(0.856)-RBS-crogfp-T
Here, pCon(J23108) constantly expressing the fusion protein crogfp(BBa_K741006) at 50% of the strength of pCon(J23100).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 124
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 918
Figure 5 shows the difference in intensity of expression of pCon(0.244) and pCon(0.856). Obviously pCon(0.856) expresses much stronger than pCon(0.244).
Data
pCon(0.856)-RBS-crogfp-T | ||||||
OD | FLU | |||||
t/min | Parallel | Experimental group | M9 medium | Parallel | Experimental group | M9 medium |
0 | 1 | 0.068 | 1 | 611.19 | ||
2 | 0.0696 | 2 | 560.005 | |||
3 | 0.0741 | 0.0416 | 3 | 540.949 | 14.037 | |
45 | 1 | 0.0787 | 1 | 625.166 | ||
2 | 0.0757 | 2 | 587.647 | |||
3 | 0.0746 | 0.0402 | 3 | 617.612 | 14.013 | |
90 | 1 | 0.0858 | 1 | 712.785 | ||
2 | 0.0827 | 2 | 673.465 | |||
3 | 0.0814 | 0.0411 | 3 | 648.529 | 14.449 | |
135 | 1 | 0.0939 | 1 | 769.154 | ||
2 | 0.0887 | 2 | 740.309 | |||
3 | 0.0877 | 0.0419 | 3 | 747.658 | 13.826 | |
180 | 1 | 0.1018 | 1 | 800.945 | ||
2 | 0.0913 | 2 | 801.508 | |||
3 | 0.0953 | 0.0396 | 3 | 862.744 | 14.123 | |
225 | 1 | 0.1182 | 1 | 903.339 | ||
2 | 0.0989 | 2 | 936.183 | |||
3 | 0.1128 | 0.0474 | 3 | 912.456 | 14.706 |