Difference between revisions of "Part:BBa K741009"

Latest revision as of 01:18, 3 October 2012

pCon(0.856)-RBS-crogfp-T

Here, pCon(J23108) constantly expressing the fusion protein crogfp(BBa_K741006) at 50% of the strength of pCon(J23100).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 124
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 918

Figure 5 shows the difference in intensity of expression of pCon(0.244) and pCon(0.856). Obviously pCon(0.856) expresses much stronger than pCon(0.244).

Data

pCon(0.856)-RBS-crogfp-T
	OD			FLU
t/min	Parallel	Experimental group	M9 medium	Parallel	Experimental group	M9 medium
0	1	0.068		1	611.19
	2	0.0696		2	560.005
	3	0.0741	0.0416	3	540.949	14.037
45	1	0.0787		1	625.166
	2	0.0757		2	587.647
	3	0.0746	0.0402	3	617.612	14.013
90	1	0.0858		1	712.785
	2	0.0827		2	673.465
	3	0.0814	0.0411	3	648.529	14.449
135	1	0.0939		1	769.154
	2	0.0887		2	740.309
	3	0.0877	0.0419	3	747.658	13.826
180	1	0.1018		1	800.945
	2	0.0913		2	801.508
	3	0.0953	0.0396	3	862.744	14.123
225	1	0.1182		1	903.339
	2	0.0989		2	936.183
	3	0.1128	0.0474	3	912.456	14.706

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K741009 short</partinfo>
-Here, pCon(J23108) constantly expressing the fusion protein crogfp at 50% of the strength of pCon(J23100).
+Here, pCon(J23108) constantly expressing the fusion protein crogfp(BBa_K741006) at 50% of the strength of pCon(J23100).
 <!-- Add more about the biology of this part here
@@ Line 16: / Line 16: @@
 ==Data==
-<table x:str border=0 cellpadding=0 cellspacing=0 width=762 style='border-collapse:
+<table x:str border=1 cellpadding=0 cellspacing=0 width=762 style='border-collapse:
   collapse;table-layout:fixed;width:572pt'>
- <col width=72 span=2 style='width:54pt'>
- <col width=166 style='mso-width-source:userset;mso-width-alt:5312;width:125pt'>
- <col width=119 style='mso-width-source:userset;mso-width-alt:3808;width:89pt'>
- <col width=82 style='mso-width-source:userset;mso-width-alt:2624;width:62pt'>
- <col width=179 style='mso-width-source:userset;mso-width-alt:5728;width:134pt'>
- <col width=72 style='width:54pt'>
   <tr height=19 style='height:14.25pt'>
    <td colspan=7 height=19 class=xl26 width=762 style='height:14.25pt;
-   width:572pt'>pCon(0.856)-RBS-crogfp-T</td>
+   width:572pt'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;pCon(0.856)-RBS-crogfp-T</td>
   </tr>
   <tr height=19 style='height:14.25pt'>
    <td height=19 class=xl27 style='height:14.25pt'>　</td>
-   <td colspan=3 class=xl27>OD</td>
+   <td colspan=3 class=xl27>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;OD</td>
-   <td colspan=3 class=xl28>FLU</td>
+   <td colspan=3 class=xl28>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;FLU</td>
   </tr>
   <tr height=19 style='height:14.25pt'>