Difference between revisions of "Part:BBa K741009"

Latest revision as of 01:18, 3 October 2012

pCon(0.856)-RBS-crogfp-T

Here, pCon(J23108) constantly expressing the fusion protein crogfp(BBa_K741006) at 50% of the strength of pCon(J23100).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 124
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 918

Figure 5 shows the difference in intensity of expression of pCon(0.244) and pCon(0.856). Obviously pCon(0.856) expresses much stronger than pCon(0.244).

Data

pCon(0.856)-RBS-crogfp-T
	OD			FLU
t/min	Parallel	Experimental group	M9 medium	Parallel	Experimental group	M9 medium
0	1	0.068		1	611.19
	2	0.0696		2	560.005
	3	0.0741	0.0416	3	540.949	14.037
45	1	0.0787		1	625.166
	2	0.0757		2	587.647
	3	0.0746	0.0402	3	617.612	14.013
90	1	0.0858		1	712.785
	2	0.0827		2	673.465
	3	0.0814	0.0411	3	648.529	14.449
135	1	0.0939		1	769.154
	2	0.0887		2	740.309
	3	0.0877	0.0419	3	747.658	13.826
180	1	0.1018		1	800.945
	2	0.0913		2	801.508
	3	0.0953	0.0396	3	862.744	14.123
225	1	0.1182		1	903.339
	2	0.0989		2	936.183
	3	0.1128	0.0474	3	912.456	14.706

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K741009 short</partinfo>
-Here, pCon(J23108) constantly expressing the fusion protein crogfp at 50% of the strength of pCon(J23100).
+Here, pCon(J23108) constantly expressing the fusion protein crogfp(BBa_K741006) at 50% of the strength of pCon(J23100).
 <!-- Add more about the biology of this part here
@@ Line 16: / Line 16: @@
 ==Data==
-<table x:str border=0 cellpadding=0 cellspacing=0 width=683 style='border-collapse:
+<table x:str border=1 cellpadding=0 cellspacing=0 width=762 style='border-collapse:
-  collapse;table-layout:fixed;width:512pt'>
+  collapse;table-layout:fixed;width:572pt'>
- <col width=72 span=6 style='width:54pt'>
- <col width=179 style='mso-width-source:userset;mso-width-alt:5728;width:134pt'>
- <col width=72 style='width:54pt'>
   <tr height=19 style='height:14.25pt'>
-   <td colspan=8 height=19 class=xl26 width=683 style='height:14.25pt;
+   <td colspan=7 height=19 class=xl26 width=762 style='height:14.25pt;
-   width:512pt'>pCon(0.856)-RBS-crogfp-T</td>
+   width:572pt'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;pCon(0.856)-RBS-crogfp-T</td>
   </tr>
   <tr height=19 style='height:14.25pt'>
    <td height=19 class=xl27 style='height:14.25pt'>　</td>
-   <td colspan=3 class=xl27>OD</td>
+   <td colspan=3 class=xl27>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;OD</td>
-  <td class=xl28>　</td>
+   <td colspan=3 class=xl28>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;FLU</td>
-   <td colspan=3 class=xl29>FLU</td>
   </tr>
   <tr height=19 style='height:14.25pt'>
    <td height=19 class=xl27 style='height:14.25pt'>t/min</td>
-   <td class=xl28>Parallel</td>
+   <td class=xl29>Parallel</td>
-   <td class=xl28>Experime<span style='display:none'>ntal group</span></td>
+   <td class=xl29>Experimental group</td>
-   <td class=xl28 colspan=2 style='mso-ignore:colspan'>M9 medium</td>
+   <td class=xl29>M9 medium</td>
-   <td class=xl28>Parallel</td>
+   <td class=xl29>Parallel</td>
-   <td class=xl28>Experimental group</td>
+   <td class=xl29>Experimental group</td>
-   <td class=xl28>M9 medium</td>
+   <td class=xl29>M9 medium</td>
   </tr>
   <tr height=19 style='height:14.25pt'>
@@ Line 44: / Line 40: @@
    <td class=xl30 x:num>1</td>
    <td class=xl31 align=right x:num="6.8000000000000005E-2">0.068</td>
-   <td colspan=2 class=xl31 style='mso-ignore:colspan'></td>
+   <td class=xl31></td>
    <td class=xl30 x:num>1</td>
    <td class=xl31 align=right x:num="611.19">611.19</td>
@@ Line 53: / Line 49: @@
    <td class=xl30 x:num>2</td>
    <td class=xl31 align=right x:num="6.9599999999999995E-2">0.0696</td>
-   <td colspan=2 class=xl31 style='mso-ignore:colspan'></td>
+   <td class=xl31></td>
    <td class=xl30 x:num>2</td>
    <td class=xl31 align=right x:num="560.005">560.005</td>
@@ Line 63: / Line 59: @@
    <td class=xl31 align=right x:num="7.4099999999999999E-2">0.0741</td>
    <td class=xl31 align=right x:num="4.1599999999999998E-2">0.0416</td>
-  <td class=xl31></td>
    <td class=xl30 x:num>3</td>
    <td class=xl31 align=right x:num="540.94899999999996">540.949</td>
@@ Line 72: / Line 67: @@
    <td class=xl30 x:num>1</td>
    <td class=xl31 align=right x:num="7.8700000000000006E-2">0.0787</td>
-   <td colspan=2 class=xl31 style='mso-ignore:colspan'></td>
+   <td class=xl31></td>
    <td class=xl30 x:num>1</td>
    <td class=xl31 align=right x:num="625.16600000000005">625.166</td>
@@ Line 81: / Line 76: @@
    <td class=xl30 x:num>2</td>
    <td class=xl31 align=right x:num="7.5700000000000003E-2">0.0757</td>
-   <td colspan=2 class=xl31 style='mso-ignore:colspan'></td>
+   <td class=xl31></td>
    <td class=xl30 x:num>2</td>
    <td class=xl31 align=right x:num="587.64700000000005">587.647</td>
@@ Line 91: / Line 86: @@
    <td class=xl31 align=right x:num="7.46E-2">0.0746</td>
    <td class=xl31 align=right x:num="4.02E-2">0.0402</td>
-  <td class=xl31></td>
    <td class=xl30 x:num>3</td>
    <td class=xl31 align=right x:num="617.61199999999997">617.612</td>
@@ Line 100: / Line 94: @@
    <td class=xl30 x:num>1</td>
    <td class=xl31 align=right x:num="8.5800000000000001E-2">0.0858</td>
-   <td colspan=2 class=xl31 style='mso-ignore:colspan'></td>
+   <td class=xl31></td>
    <td class=xl30 x:num>1</td>
    <td class=xl31 align=right x:num="712.78499999999997">712.785</td>
@@ Line 109: / Line 103: @@
    <td class=xl30 x:num>2</td>
    <td class=xl31 align=right x:num="8.2699999999999996E-2">0.0827</td>
-   <td colspan=2 class=xl31 style='mso-ignore:colspan'></td>
+   <td class=xl31></td>
    <td class=xl30 x:num>2</td>
    <td class=xl31 align=right x:num="673.46500000000003">673.465</td>
@@ Line 119: / Line 113: @@
    <td class=xl31 align=right x:num="8.14E-2">0.0814</td>
    <td class=xl31 align=right x:num="4.1099999999999998E-2">0.0411</td>
-  <td class=xl31></td>
    <td class=xl30 x:num>3</td>
    <td class=xl31 align=right x:num="648.529">648.529</td>
@@ Line 128: / Line 121: @@
    <td class=xl30 x:num>1</td>
    <td class=xl31 align=right x:num="9.3899999999999997E-2">0.0939</td>
-   <td colspan=2 class=xl31 style='mso-ignore:colspan'></td>
+   <td class=xl31></td>
    <td class=xl30 x:num>1</td>
    <td class=xl31 align=right x:num="769.154">769.154</td>
@@ Line 137: / Line 130: @@
    <td class=xl30 x:num>2</td>
    <td class=xl31 align=right x:num="8.8700000000000001E-2">0.0887</td>
-   <td colspan=2 class=xl31 style='mso-ignore:colspan'></td>
+   <td class=xl31></td>
    <td class=xl30 x:num>2</td>
    <td class=xl31 align=right x:num="740.30899999999997">740.309</td>
@@ Line 147: / Line 140: @@
    <td class=xl31 align=right x:num="8.77E-2">0.0877</td>
    <td class=xl31 align=right x:num="4.19E-2">0.0419</td>
-  <td class=xl31></td>
    <td class=xl30 x:num>3</td>
    <td class=xl31 align=right x:num="747.65800000000002">747.658</td>
@@ Line 156: / Line 148: @@
    <td class=xl30 x:num>1</td>
    <td class=xl31 align=right x:num="0.1018">0.1018</td>
-   <td colspan=2 class=xl31 style='mso-ignore:colspan'></td>
+   <td class=xl31></td>
    <td class=xl30 x:num>1</td>
    <td class=xl31 align=right x:num="800.94500000000005">800.945</td>
@@ Line 165: / Line 157: @@
    <td class=xl30 x:num>2</td>
    <td class=xl31 align=right x:num="9.1300000000000006E-2">0.0913</td>
-   <td colspan=2 class=xl31 style='mso-ignore:colspan'></td>
+   <td class=xl31></td>
    <td class=xl30 x:num>2</td>
    <td class=xl31 align=right x:num="801.50800000000004">801.508</td>
@@ Line 175: / Line 167: @@
    <td class=xl31 align=right x:num="9.5299999999999996E-2">0.0953</td>
    <td class=xl31 align=right x:num="3.9600000000000003E-2">0.0396</td>
-  <td class=xl31></td>
    <td class=xl30 x:num>3</td>
    <td class=xl31 align=right x:num="862.74400000000003">862.744</td>
@@ Line 184: / Line 175: @@
    <td class=xl30 x:num>1</td>
    <td class=xl31 align=right x:num="0.1182">0.1182</td>
-   <td colspan=2 class=xl31 style='mso-ignore:colspan'></td>
+   <td class=xl31></td>
    <td class=xl30 x:num>1</td>
    <td class=xl31 align=right x:num="903.33900000000006">903.339</td>
@@ Line 193: / Line 184: @@
    <td class=xl30 x:num>2</td>
    <td class=xl31 align=right x:num="9.8900000000000002E-2">0.0989</td>
-   <td colspan=2 class=xl31 style='mso-ignore:colspan'></td>
+   <td class=xl31></td>
    <td class=xl30 x:num>2</td>
    <td class=xl31 align=right x:num="936.18299999999999">936.183</td>
@@ Line 203: / Line 194: @@
    <td class=xl31 align=right x:num="0.1128">0.1128</td>
    <td class=xl31 align=right x:num="4.7399999999999998E-2">0.0474</td>
-  <td class=xl31></td>
    <td class=xl30 x:num>3</td>
    <td class=xl31 align=right x:num="912.45600000000002">912.456</td>