Difference between revisions of "Part:BBa K741009"

 
 
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__NOTOC__
 
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<partinfo>BBa_K741009 short</partinfo>
 
<partinfo>BBa_K741009 short</partinfo>
  
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Here, pCon(J23108) constantly expressing the fusion protein crogfp at 50% of the strength of pCon(J23100).  
+
Here, pCon(J23108) constantly expressing the fusion protein crogfp(BBa_K741006) at 50% of the strength of pCon(J23100).  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
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<partinfo>BBa_K741009 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K741009 SequenceAndFeatures</partinfo>
  
 +
[[Image:USTC2012Result5_副本.png]]
 +
<p>Figure 5 shows the difference in intensity of expression of pCon(0.244) and pCon(0.856). Obviously pCon(0.856) expresses much stronger than pCon(0.244).</p>
 +
 +
==Data==
  
 +
<table x:str border=1 cellpadding=0 cellspacing=0 width=762 style='border-collapse:
 +
collapse;table-layout:fixed;width:572pt'>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td colspan=7 height=19 class=xl26 width=762 style='height:14.25pt;
 +
  width:572pt'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;pCon(0.856)-RBS-crogfp-T</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt'>　</td>
 +
  <td colspan=3 class=xl27>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;OD</td>
 +
  <td colspan=3 class=xl28>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;FLU</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl27 style='height:14.25pt'>t/min</td>
 +
  <td class=xl29>Parallel</td>
 +
  <td class=xl29>Experimental group</td>
 +
  <td class=xl29>M9 medium</td>
 +
  <td class=xl29>Parallel</td>
 +
  <td class=xl29>Experimental group</td>
 +
  <td class=xl29>M9 medium</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl30 style='height:14.25pt' x:num>0</td>
 +
  <td class=xl30 x:num>1</td>
 +
  <td class=xl31 align=right x:num="6.8000000000000005E-2">0.068</td>
 +
  <td class=xl31></td>
 +
  <td class=xl30 x:num>1</td>
 +
  <td class=xl31 align=right x:num="611.19">611.19</td>
 +
  <td class=xl31></td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl30 style='height:14.25pt'></td>
 +
  <td class=xl30 x:num>2</td>
 +
  <td class=xl31 align=right x:num="6.9599999999999995E-2">0.0696</td>
 +
  <td class=xl31></td>
 +
  <td class=xl30 x:num>2</td>
 +
  <td class=xl31 align=right x:num="560.005">560.005</td>
 +
  <td class=xl31></td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl30 style='height:14.25pt'></td>
 +
  <td class=xl30 x:num>3</td>
 +
  <td class=xl31 align=right x:num="7.4099999999999999E-2">0.0741</td>
 +
  <td class=xl31 align=right x:num="4.1599999999999998E-2">0.0416</td>
 +
  <td class=xl30 x:num>3</td>
 +
  <td class=xl31 align=right x:num="540.94899999999996">540.949</td>
 +
  <td class=xl31 align=right x:num="14.037000000000001">14.037</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl30 style='height:14.25pt' x:num>45</td>
 +
  <td class=xl30 x:num>1</td>
 +
  <td class=xl31 align=right x:num="7.8700000000000006E-2">0.0787</td>
 +
  <td class=xl31></td>
 +
  <td class=xl30 x:num>1</td>
 +
  <td class=xl31 align=right x:num="625.16600000000005">625.166</td>
 +
  <td class=xl31></td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl30 style='height:14.25pt'></td>
 +
  <td class=xl30 x:num>2</td>
 +
  <td class=xl31 align=right x:num="7.5700000000000003E-2">0.0757</td>
 +
  <td class=xl31></td>
 +
  <td class=xl30 x:num>2</td>
 +
  <td class=xl31 align=right x:num="587.64700000000005">587.647</td>
 +
  <td class=xl31></td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl30 style='height:14.25pt'></td>
 +
  <td class=xl30 x:num>3</td>
 +
  <td class=xl31 align=right x:num="7.46E-2">0.0746</td>
 +
  <td class=xl31 align=right x:num="4.02E-2">0.0402</td>
 +
  <td class=xl30 x:num>3</td>
 +
  <td class=xl31 align=right x:num="617.61199999999997">617.612</td>
 +
  <td class=xl31 align=right x:num="14.013">14.013</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl30 style='height:14.25pt' x:num>90</td>
 +
  <td class=xl30 x:num>1</td>
 +
  <td class=xl31 align=right x:num="8.5800000000000001E-2">0.0858</td>
 +
  <td class=xl31></td>
 +
  <td class=xl30 x:num>1</td>
 +
  <td class=xl31 align=right x:num="712.78499999999997">712.785</td>
 +
  <td class=xl31></td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl30 style='height:14.25pt'></td>
 +
  <td class=xl30 x:num>2</td>
 +
  <td class=xl31 align=right x:num="8.2699999999999996E-2">0.0827</td>
 +
  <td class=xl31></td>
 +
  <td class=xl30 x:num>2</td>
 +
  <td class=xl31 align=right x:num="673.46500000000003">673.465</td>
 +
  <td class=xl31></td>
 +
</tr>
 +
<tr height=17 style='mso-height-source:userset;height:12.75pt'>
 +
  <td height=17 class=xl30 style='height:12.75pt'></td>
 +
  <td class=xl30 x:num>3</td>
 +
  <td class=xl31 align=right x:num="8.14E-2">0.0814</td>
 +
  <td class=xl31 align=right x:num="4.1099999999999998E-2">0.0411</td>
 +
  <td class=xl30 x:num>3</td>
 +
  <td class=xl31 align=right x:num="648.529">648.529</td>
 +
  <td class=xl31 align=right x:num="14.449">14.449</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl30 style='height:14.25pt' x:num>135</td>
 +
  <td class=xl30 x:num>1</td>
 +
  <td class=xl31 align=right x:num="9.3899999999999997E-2">0.0939</td>
 +
  <td class=xl31></td>
 +
  <td class=xl30 x:num>1</td>
 +
  <td class=xl31 align=right x:num="769.154">769.154</td>
 +
  <td class=xl31></td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl30 style='height:14.25pt'></td>
 +
  <td class=xl30 x:num>2</td>
 +
  <td class=xl31 align=right x:num="8.8700000000000001E-2">0.0887</td>
 +
  <td class=xl31></td>
 +
  <td class=xl30 x:num>2</td>
 +
  <td class=xl31 align=right x:num="740.30899999999997">740.309</td>
 +
  <td class=xl31></td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl30 style='height:14.25pt'></td>
 +
  <td class=xl30 x:num>3</td>
 +
  <td class=xl31 align=right x:num="8.77E-2">0.0877</td>
 +
  <td class=xl31 align=right x:num="4.19E-2">0.0419</td>
 +
  <td class=xl30 x:num>3</td>
 +
  <td class=xl31 align=right x:num="747.65800000000002">747.658</td>
 +
  <td class=xl31 align=right x:num="13.826000000000001">13.826</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl30 style='height:14.25pt' x:num>180</td>
 +
  <td class=xl30 x:num>1</td>
 +
  <td class=xl31 align=right x:num="0.1018">0.1018</td>
 +
  <td class=xl31></td>
 +
  <td class=xl30 x:num>1</td>
 +
  <td class=xl31 align=right x:num="800.94500000000005">800.945</td>
 +
  <td class=xl31></td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl30 style='height:14.25pt'></td>
 +
  <td class=xl30 x:num>2</td>
 +
  <td class=xl31 align=right x:num="9.1300000000000006E-2">0.0913</td>
 +
  <td class=xl31></td>
 +
  <td class=xl30 x:num>2</td>
 +
  <td class=xl31 align=right x:num="801.50800000000004">801.508</td>
 +
  <td class=xl31></td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl30 style='height:14.25pt'></td>
 +
  <td class=xl30 x:num>3</td>
 +
  <td class=xl31 align=right x:num="9.5299999999999996E-2">0.0953</td>
 +
  <td class=xl31 align=right x:num="3.9600000000000003E-2">0.0396</td>
 +
  <td class=xl30 x:num>3</td>
 +
  <td class=xl31 align=right x:num="862.74400000000003">862.744</td>
 +
  <td class=xl31 align=right x:num="14.122999999999999">14.123</td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl30 style='height:14.25pt' x:num>225</td>
 +
  <td class=xl30 x:num>1</td>
 +
  <td class=xl31 align=right x:num="0.1182">0.1182</td>
 +
  <td class=xl31></td>
 +
  <td class=xl30 x:num>1</td>
 +
  <td class=xl31 align=right x:num="903.33900000000006">903.339</td>
 +
  <td class=xl31></td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl30 style='height:14.25pt'></td>
 +
  <td class=xl30 x:num>2</td>
 +
  <td class=xl31 align=right x:num="9.8900000000000002E-2">0.0989</td>
 +
  <td class=xl31></td>
 +
  <td class=xl30 x:num>2</td>
 +
  <td class=xl31 align=right x:num="936.18299999999999">936.183</td>
 +
  <td class=xl31></td>
 +
</tr>
 +
<tr height=19 style='height:14.25pt'>
 +
  <td height=19 class=xl30 style='height:14.25pt'></td>
 +
  <td class=xl30 x:num>3</td>
 +
  <td class=xl31 align=right x:num="0.1128">0.1128</td>
 +
  <td class=xl31 align=right x:num="4.7399999999999998E-2">0.0474</td>
 +
  <td class=xl30 x:num>3</td>
 +
  <td class=xl31 align=right x:num="912.45600000000002">912.456</td>
 +
  <td class=xl31 align=right x:num="14.706">14.706</td>
 +
</tr>
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K741009 parameters</partinfo>
 
<partinfo>BBa_K741009 parameters</partinfo>
 
<!-- -->
 
<!-- -->

Latest revision as of 01:18, 3 October 2012

pCon(0.856)-RBS-crogfp-T

Here, pCon(J23108) constantly expressing the fusion protein crogfp(BBa_K741006) at 50% of the strength of pCon(J23100).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 124
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 918

USTC2012Result5 副本.png

Figure 5 shows the difference in intensity of expression of pCon(0.244) and pCon(0.856). Obviously pCon(0.856) expresses much stronger than pCon(0.244).

Data

                                         pCon(0.856)-RBS-crogfp-T
　                   OD                   FLU
t/min Parallel Experimental group M9 medium Parallel Experimental group M9 medium
0 1 0.068 1 611.19
2 0.0696 2 560.005
3 0.0741 0.0416 3 540.949 14.037
45 1 0.0787 1 625.166
2 0.0757 2 587.647
3 0.0746 0.0402 3 617.612 14.013
90 1 0.0858 1 712.785
2 0.0827 2 673.465
3 0.0814 0.0411 3 648.529 14.449
135 1 0.0939 1 769.154
2 0.0887 2 740.309
3 0.0877 0.0419 3 747.658 13.826
180 1 0.1018 1 800.945
2 0.0913 2 801.508
3 0.0953 0.0396 3 862.744 14.123
225 1 0.1182 1 903.339
2 0.0989 2 936.183
3 0.1128 0.0474 3 912.456 14.706