Difference between revisions of "Part:BBa K741008"

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<partinfo>BBa_K741008 short</partinfo>
 
<partinfo>BBa_K741008 short</partinfo>
  
−
Here, pCon(J23114) constantly expressing the fusion protein crogfp at 10% of the strength of pCon(J23100).
+
Here, pCon(J23114) constantly expressing the fusion protein crogfp(BBa_K741006) at 10% of the strength of pCon(J23100).
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 19: Line 19:
 
  <tr height=19 style='height:14.25pt'>
 
  <tr height=19 style='height:14.25pt'>
 
   <td colspan=7 height=19 class=xl24 width=742 style='height:14.25pt;
 
   <td colspan=7 height=19 class=xl24 width=742 style='height:14.25pt;
−
   width:556pt'>pCon(0.244)-RBS-crogfp-T</td>
+
   width:556pt'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;pCon(0.244)-RBS-crogfp-T</td>
 
  </tr>
 
  </tr>
 
  <tr height=19 style='height:14.25pt'>
 
  <tr height=19 style='height:14.25pt'>
−
   <td height=19 class=xl25 style='height:14.25pt'></td>
+
   <td height=19 class=xl25 style='height:14.25pt;border-top:none'>　</td>
−
   <td colspan=3 class=xl24>OD</td>
+
   <td colspan=3 class=xl24 style='border-left:none'>OD</td>
−
   <td colspan=3 class=xl24>fluorescence intensity</td>
+
   <td colspan=3 class=xl24 style='border-left:none'>fluorescence intensity</td>
 
  </tr>
 
  </tr>
 
  <tr height=19 style='height:14.25pt'>
 
  <tr height=19 style='height:14.25pt'>
−
   <td height=19 class=xl25 style='height:14.25pt'>t/min</td>
+
   <td height=19 class=xl25 style='height:14.25pt;border-top:none'>t/min</td>
−
   <td class=xl25 x:str="Parellel ">Parellel<span
+
   <td class=xl25 style='border-top:none;border-left:none' x:str="Parallel ">Parallel<span
 
   style='mso-spacerun:yes'>&nbsp;</span></td>
 
   style='mso-spacerun:yes'>&nbsp;</span></td>
−
   <td class=xl25>Experimental group</td>
+
   <td class=xl25 style='border-top:none;border-left:none'>Experimental group</td>
−
   <td class=xl25>M9 medium</td>
+
   <td class=xl25 style='border-top:none;border-left:none'>M9 medium</td>
−
   <td class=xl25 x:str="Parellel ">Parellel<span
+
   <td class=xl25 style='border-top:none;border-left:none' x:str="Parallel ">Parallel<span
 
   style='mso-spacerun:yes'>&nbsp;</span></td>
 
   style='mso-spacerun:yes'>&nbsp;</span></td>
−
   <td class=xl25>Experimental group</td>
+
   <td class=xl25 style='border-top:none;border-left:none'>Experimental group</td>
−
   <td class=xl25>M9 medium</td>
+
   <td class=xl25 style='border-top:none;border-left:none'>M9 medium</td>
 
  </tr>
 
  </tr>
 
  <tr height=19 style='height:14.25pt'>
 
  <tr height=19 style='height:14.25pt'>
−
   <td height=19 class=xl25 align=right style='height:14.25pt' x:num>0</td>
+
   <td height=19 class=xl25 align=right style='height:14.25pt;border-top:none'
−
   <td class=xl25 align=right x:num>1</td>
+
  x:num>0</td>
−
   <td class=xl25 align=right x:num="0.13600000000000001">0.136</td>
+
   <td class=xl25 align=right style='border-top:none;border-left:none' x:num>1</td>
−
   <td class=xl25 align=right x:num="5.5899999999999998E-2">0.0559</td>
+
   <td class=xl25 align=right style='border-top:none;border-left:none'
−
   <td class=xl25 align=right x:num>1</td>
+
  x:num="0.13600000000000001">0.136</td>
−
   <td class=xl25 align=right x:num="132.35499999999999">132.355</td>
+
   <td class=xl25 align=right style='border-top:none;border-left:none'
−
   <td class=xl25 align=right x:num="18.094999999999999">18.095</td>
+
  x:num="5.5899999999999998E-2">0.0559</td>
 +
   <td class=xl25 align=right style='border-top:none;border-left:none' x:num>1</td>
 +
   <td class=xl25 align=right style='border-top:none;border-left:none'
 +
  x:num="132.35499999999999">132.355</td>
 +
   <td class=xl25 align=right style='border-top:none;border-left:none'
 +
  x:num="18.094999999999999">18.095</td>
 
  </tr>
 
  </tr>
 
  <tr height=19 style='height:14.25pt'>
 
  <tr height=19 style='height:14.25pt'>
−
   <td height=19 class=xl25 style='height:14.25pt'></td>
+
   <td height=19 class=xl25 style='height:14.25pt;border-top:none'>　</td>
−
   <td class=xl25 align=right x:num>2</td>
+
   <td class=xl25 align=right style='border-top:none;border-left:none' x:num>2</td>
−
   <td class=xl25 align=right x:num="0.1313">0.1313</td>
+
   <td class=xl25 align=right style='border-top:none;border-left:none'
−
   <td class=xl25></td>
+
  x:num="0.1313">0.1313</td>
−
   <td class=xl25 align=right x:num>2</td>
+
   <td class=xl25 style='border-top:none;border-left:none'>　</td>
−
   <td class=xl25 align=right x:num="116.709">116.709</td>
+
   <td class=xl25 align=right style='border-top:none;border-left:none' x:num>2</td>
−
   <td class=xl25></td>
+
   <td class=xl25 align=right style='border-top:none;border-left:none'
 +
  x:num="116.709">116.709</td>
 +
   <td class=xl25 style='border-top:none;border-left:none'>　</td>
 
  </tr>
 
  </tr>
 
  <tr height=19 style='height:14.25pt'>
 
  <tr height=19 style='height:14.25pt'>
−
   <td height=19 colspan=7 class=xl25 style='height:14.25pt;mso-ignore:colspan'></td>
+
   <td height=19 class=xl25 align=right style='height:14.25pt;border-top:none'
 +
  x:num>45</td>
 +
  <td class=xl25 align=right style='border-top:none;border-left:none' x:num>1</td>
 +
  <td class=xl25 align=right style='border-top:none;border-left:none'
 +
  x:num="0.1988">0.1988</td>
 +
  <td class=xl25 align=right style='border-top:none;border-left:none'
 +
  x:num="5.2200000000000003E-2">0.0522</td>
 +
  <td class=xl25 align=right style='border-top:none;border-left:none' x:num>1</td>
 +
  <td class=xl25 align=right style='border-top:none;border-left:none'
 +
  x:num="180.53700000000001">180.537</td>
 +
  <td class=xl25 align=right style='border-top:none;border-left:none'
 +
  x:num="19.878">19.878</td>
 
  </tr>
 
  </tr>
 
  <tr height=19 style='height:14.25pt'>
 
  <tr height=19 style='height:14.25pt'>
−
   <td height=19 class=xl25 align=right style='height:14.25pt' x:num>45</td>
+
   <td height=19 class=xl25 style='height:14.25pt;border-top:none'>　</td>
−
   <td class=xl25 align=right x:num>1</td>
+
   <td class=xl25 align=right style='border-top:none;border-left:none' x:num>2</td>
−
   <td class=xl25 align=right x:num="0.1988">0.1988</td>
+
   <td class=xl25 align=right style='border-top:none;border-left:none'
−
   <td class=xl25 align=right x:num="5.2200000000000003E-2">0.0522</td>
+
  x:num="0.20039999999999999">0.2004</td>
−
   <td class=xl25 align=right x:num>1</td>
+
   <td class=xl25 style='border-top:none;border-left:none'>　</td>
−
   <td class=xl25 align=right x:num="180.53700000000001">180.537</td>
+
   <td class=xl25 align=right style='border-top:none;border-left:none' x:num>2</td>
−
   <td class=xl25 align=right x:num="19.878">19.878</td>
+
   <td class=xl25 align=right style='border-top:none;border-left:none'
 +
  x:num="172.49700000000001">172.497</td>
 +
   <td class=xl25 style='border-top:none;border-left:none'>　</td>
 
  </tr>
 
  </tr>
 
  <tr height=19 style='height:14.25pt'>
 
  <tr height=19 style='height:14.25pt'>
−
   <td height=19 class=xl25 style='height:14.25pt'></td>
+
   <td height=19 class=xl25 align=right style='height:14.25pt;border-top:none'
−
   <td class=xl25 align=right x:num>2</td>
+
  x:num>90</td>
−
   <td class=xl25 align=right x:num="0.20039999999999999">0.2004</td>
+
   <td class=xl25 align=right style='border-top:none;border-left:none' x:num>1</td>
−
   <td class=xl25></td>
+
   <td class=xl25 align=right style='border-top:none;border-left:none'
−
   <td class=xl25 align=right x:num>2</td>
+
  x:num="0.31530000000000002">0.3153</td>
−
   <td class=xl25 align=right x:num="172.49700000000001">172.497</td>
+
   <td class=xl25 align=right style='border-top:none;border-left:none'
−
   <td class=xl25></td>
+
  x:num="5.4300000000000001E-2">0.0543</td>
 +
   <td class=xl25 align=right style='border-top:none;border-left:none' x:num>1</td>
 +
   <td class=xl25 align=right style='border-top:none;border-left:none'
 +
  x:num="267.399">267.399</td>
 +
   <td class=xl25 align=right style='border-top:none;border-left:none'
 +
  x:num="21.225000000000001">21.225</td>
 
  </tr>
 
  </tr>
 
  <tr height=19 style='height:14.25pt'>
 
  <tr height=19 style='height:14.25pt'>
−
   <td height=19 colspan=7 class=xl25 style='height:14.25pt;mso-ignore:colspan'></td>
+
   <td height=19 class=xl25 style='height:14.25pt;border-top:none'>　</td>
 +
  <td class=xl25 align=right style='border-top:none;border-left:none' x:num>2</td>
 +
  <td class=xl25 align=right style='border-top:none;border-left:none'
 +
  x:num="0.31259999999999999">0.3126</td>
 +
  <td class=xl25 style='border-top:none;border-left:none'>　</td>
 +
  <td class=xl25 align=right style='border-top:none;border-left:none' x:num>2</td>
 +
  <td class=xl25 align=right style='border-top:none;border-left:none'
 +
  x:num="243.57900000000001">243.579</td>
 +
  <td class=xl25 style='border-top:none;border-left:none'>　</td>
 
  </tr>
 
  </tr>
 
  <tr height=19 style='height:14.25pt'>
 
  <tr height=19 style='height:14.25pt'>
−
   <td height=19 class=xl25 align=right style='height:14.25pt' x:num>90</td>
+
   <td height=19 class=xl25 align=right style='height:14.25pt;border-top:none'
−
   <td class=xl25 align=right x:num>1</td>
+
  x:num>135</td>
−
   <td class=xl25 align=right x:num="0.31530000000000002">0.3153</td>
+
   <td class=xl25 align=right style='border-top:none;border-left:none' x:num>1</td>
−
   <td class=xl25 align=right x:num="5.4300000000000001E-2">0.0543</td>
+
   <td class=xl25 align=right style='border-top:none;border-left:none'
−
   <td class=xl25 align=right x:num>1</td>
+
  x:num="0.44500000000000001">0.445</td>
−
   <td class=xl25 align=right x:num="267.399">267.399</td>
+
   <td class=xl25 align=right style='border-top:none;border-left:none'
−
   <td class=xl25 align=right x:num="21.225000000000001">21.225</td>
+
  x:num="5.4100000000000002E-2">0.0541</td>
 +
   <td class=xl25 align=right style='border-top:none;border-left:none' x:num>1</td>
 +
   <td class=xl25 align=right style='border-top:none;border-left:none'
 +
  x:num="346.81400000000002">346.814</td>
 +
   <td class=xl25 align=right style='border-top:none;border-left:none'
 +
  x:num="20.907">20.907</td>
 
  </tr>
 
  </tr>
 
  <tr height=19 style='height:14.25pt'>
 
  <tr height=19 style='height:14.25pt'>
−
   <td height=19 class=xl25 style='height:14.25pt'></td>
+
   <td height=19 class=xl25 style='height:14.25pt;border-top:none'>　</td>
−
   <td class=xl25 align=right x:num>2</td>
+
   <td class=xl25 align=right style='border-top:none;border-left:none' x:num>2</td>
−
   <td class=xl25 align=right x:num="0.31259999999999999">0.3126</td>
+
   <td class=xl25 align=right style='border-top:none;border-left:none'
−
   <td class=xl25></td>
+
  x:num="0.44779999999999998">0.4478</td>
−
   <td class=xl25 align=right x:num>2</td>
+
   <td class=xl25 style='border-top:none;border-left:none'>　</td>
−
   <td class=xl25 align=right x:num="243.57900000000001">243.579</td>
+
   <td class=xl25 align=right style='border-top:none;border-left:none' x:num>2</td>
−
   <td class=xl25></td>
+
   <td class=xl25 align=right style='border-top:none;border-left:none'
 +
  x:num="357.69600000000003">357.696</td>
 +
   <td class=xl25 style='border-top:none;border-left:none'>　</td>
 
  </tr>
 
  </tr>
 
  <tr height=19 style='height:14.25pt'>
 
  <tr height=19 style='height:14.25pt'>
−
   <td height=19 colspan=7 class=xl25 style='height:14.25pt;mso-ignore:colspan'></td>
+
   <td height=19 class=xl25 align=right style='height:14.25pt;border-top:none'
 +
  x:num>180</td>
 +
  <td class=xl25 align=right style='border-top:none;border-left:none' x:num>1</td>
 +
  <td class=xl25 align=right style='border-top:none;border-left:none'
 +
  x:num="0.49490000000000001">0.4949</td>
 +
  <td class=xl25 align=right style='border-top:none;border-left:none'
 +
  x:num="5.33E-2">0.0533</td>
 +
  <td class=xl25 align=right style='border-top:none;border-left:none' x:num>1</td>
 +
  <td class=xl25 align=right style='border-top:none;border-left:none'
 +
  x:num="423.673">423.673</td>
 +
  <td class=xl25 align=right style='border-top:none;border-left:none'
 +
  x:num="21.545000000000002">21.545</td>
 
  </tr>
 
  </tr>
 
  <tr height=19 style='height:14.25pt'>
 
  <tr height=19 style='height:14.25pt'>
−
   <td height=19 class=xl25 align=right style='height:14.25pt' x:num>135</td>
+
   <td height=19 class=xl25 style='height:14.25pt;border-top:none'>　</td>
−
   <td class=xl25 align=right x:num>1</td>
+
   <td class=xl25 align=right style='border-top:none;border-left:none' x:num>2</td>
−
  <td class=xl25 align=right x:num="0.44500000000000001">0.445</td>
+
   <td class=xl25 align=right style='border-top:none;border-left:none'
−
  <td class=xl25 align=right x:num="5.4100000000000002E-2">0.0541</td>
+
   x:num="0.50139999999999996">0.5014</td>
−
  <td class=xl25 align=right x:num>1</td>
+
   <td class=xl25 style='border-top:none;border-left:none'>　</td>
−
  <td class=xl25 align=right x:num="346.81400000000002">346.814</td>
+
   <td class=xl25 align=right style='border-top:none;border-left:none' x:num>2</td>
−
   <td class=xl25 align=right x:num="20.907">20.907</td>
+
   <td class=xl25 align=right style='border-top:none;border-left:none'
−
</tr>
+
   x:num="454.13600000000002">454.136</td>
−
<tr height=19 style='height:14.25pt'>
+
   <td class=xl25 style='border-top:none;border-left:none'>　</td>
−
  <td height=19 class=xl25 style='height:14.25pt'></td>
+
−
   <td class=xl25 align=right x:num>2</td>
+
−
  <td class=xl25 align=right x:num="0.44779999999999998">0.4478</td>
+
−
   <td class=xl25></td>
+
−
  <td class=xl25 align=right x:num>2</td>
+
−
  <td class=xl25 align=right x:num="357.69600000000003">357.696</td>
+
−
  <td class=xl25></td>
+
−
</tr>
+
−
<tr height=19 style='height:14.25pt'>
+
−
  <td height=19 colspan=7 class=xl25 style='height:14.25pt;mso-ignore:colspan'></td>
+
−
</tr>
+
−
<tr height=19 style='height:14.25pt'>
+
−
   <td height=19 class=xl25 align=right style='height:14.25pt' x:num>180</td>
+
−
  <td class=xl25 align=right x:num>1</td>
+
−
  <td class=xl25 align=right x:num="0.49490000000000001">0.4949</td>
+
−
  <td class=xl25 align=right x:num="5.33E-2">0.0533</td>
+
−
   <td class=xl25 align=right x:num>1</td>
+
−
  <td class=xl25 align=right x:num="423.673">423.673</td>
+
−
  <td class=xl25 align=right x:num="21.545000000000002">21.545</td>
+
−
</tr>
+
−
<tr height=19 style='height:14.25pt'>
+
−
  <td height=19 class=xl25 style='height:14.25pt'></td>
+
−
   <td class=xl25 align=right x:num>2</td>
+
−
  <td class=xl25 align=right x:num="0.50139999999999996">0.5014</td>
+
−
  <td class=xl25></td>
+
−
  <td class=xl25 align=right x:num>2</td>
+
−
  <td class=xl25 align=right x:num="454.13600000000002">454.136</td>
+
−
   <td class=xl25></td>
+
 
  </tr>
 
  </tr>
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 01:17, 3 October 2012

pCon(0.244)-RBS-crogfp-T

Here, pCon(J23114) constantly expressing the fusion protein crogfp(BBa_K741006) at 10% of the strength of pCon(J23100).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 124
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 918

USTC2012Result5 副本.png

Figure 5 shows the difference in intensity of expression of pCon(0.244) and pCon(0.856). Obviously pCon(0.856) expresses much stronger than pCon(0.244).

Data

                                            pCon(0.244)-RBS-crogfp-T
　 OD fluorescence intensity
t/min Parallel  Experimental group M9 medium Parallel  Experimental group M9 medium
0 1 0.136 0.0559 1 132.355 18.095
　 2 0.1313 　 2 116.709 　
45 1 0.1988 0.0522 1 180.537 19.878
　 2 0.2004 　 2 172.497 　
90 1 0.3153 0.0543 1 267.399 21.225
　 2 0.3126 　 2 243.579 　
135 1 0.445 0.0541 1 346.814 20.907
　 2 0.4478 　 2 357.696 　
180 1 0.4949 0.0533 1 423.673 21.545
　 2 0.5014 　 2 454.136