Difference between revisions of "Part:BBa K741001"

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<partinfo>BBa_K741001 short</partinfo>
 
<partinfo>BBa_K741001 short</partinfo>
  
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It can generates protein CI whereas the expression can only be started when the promoter plac is activated by IPTG.
+
Lac promoter with cI gene downstream. LacI or glucose can repress the expression of cI while lactose and IPTG can activates it.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K741001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K741001 SequenceAndFeatures</partinfo>
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−
 
−
== Data ==
 
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<p> Fluorescence intensity </p>
 
−
 
−
 
−
 
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<table x:str border=1 cellpadding=0 cellspacing=0 width=1224 style='border-collapse:
 
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collapse;table-layout:fixed;width:918pt align:center;'>
 
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<col width=72 span=17 style='width:54pt'>
 
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<tr height=19 style='height:14.25pt'>
 
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  <td colspan=17 height=19 class=xl32 width=1224 style='border-right:.5pt solid black;
 
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  height:14.25pt;width:918pt'>Fluorescence intensity</td>
 
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</tr>
 
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<tr height=19 style='height:14.25pt'>
 
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  <td colspan=17 height=19 class=xl35 style='border-right:.5pt solid black;
 
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  height:14.25pt'>plac-RBS-GFP-T</td>
 
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</tr>
 
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<tr height=19 style='height:14.25pt'>
 
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  <td height=19 class=xl38 style='height:14.25pt;border-top:none'>t/min</td>
 
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  <td class=xl39 style='border-top:none;border-left:none'>M9 medium</td>
 
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  <td class=xl40 style='border-top:none;border-left:none'>　</td>
 
−
  <td class=xl40 style='border-top:none;border-left:none' x:num>0</td>
 
−
  <td class=xl40 style='border-top:none;border-left:none'>　</td>
 
−
  <td class=xl41 style='border-top:none;border-left:none'>　</td>
 
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  <td class=xl42 style='border-top:none;border-left:none'>500mM glucose</td>
 
−
  <td class=xl41 style='border-top:none;border-left:none'>　</td>
 
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  <td class=xl43 style='border-top:none;border-left:none'>　</td>
 
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  <td class=xl44 style='border-top:none;border-left:none'>800mM glucose</td>
 
−
  <td class=xl43 style='border-top:none;border-left:none'>　</td>
 
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  <td class=xl45 style='border-top:none;border-left:none'>　</td>
 
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  <td class=xl45 style='border-top:none;border-left:none'>1000mM glucose</td>
 
−
  <td class=xl45 style='border-top:none;border-left:none'>　</td>
 
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  <td class=xl46 style='border-top:none;border-left:none'>　</td>
 
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  <td class=xl46 style='border-top:none;border-left:none'>1000mM IPTG</td>
 
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  <td class=xl46 style='border-top:none;border-left:none'>　</td>
 
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</tr>
 
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<tr height=19 style='height:14.25pt'>
 
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  <td height=19 class=xl38 style='height:14.25pt;border-top:none'>Parallel</td>
 
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  <td class=xl39 style='border-top:none;border-left:none' x:num>1</td>
 
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  <td class=xl40 style='border-top:none;border-left:none' x:num>1</td>
 
−
  <td class=xl40 style='border-top:none;border-left:none' x:num>2</td>
 
−
  <td class=xl40 style='border-top:none;border-left:none' x:num>3</td>
 
−
  <td class=xl41 style='border-top:none;border-left:none' x:num>1</td>
 
−
  <td class=xl42 style='border-top:none;border-left:none' x:num>2</td>
 
−
  <td class=xl41 style='border-top:none;border-left:none' x:num>3</td>
 
−
  <td class=xl43 style='border-top:none;border-left:none' x:num>1</td>
 
−
  <td class=xl44 style='border-top:none;border-left:none' x:num>2</td>
 
−
  <td class=xl43 style='border-top:none;border-left:none' x:num>3</td>
 
−
  <td class=xl45 style='border-top:none;border-left:none' x:num>1</td>
 
−
  <td class=xl45 style='border-top:none;border-left:none' x:num>2</td>
 
−
  <td class=xl45 style='border-top:none;border-left:none' x:num>3</td>
 
−
  <td class=xl46 style='border-top:none;border-left:none' x:num>1</td>
 
−
  <td class=xl46 style='border-top:none;border-left:none' x:num>2</td>
 
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  <td class=xl46 style='border-top:none;border-left:none' x:num>3</td>
 
−
</tr>
 
−
<tr height=19 style='height:14.25pt'>
 
−
  <td height=19 class=xl47 style='height:14.25pt;border-top:none' x:num>0</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none' x:num="11.801">11.801</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none' x:num="197.047">197.047</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none' x:num="198.876">198.876</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none' x:num="190.953">190.953</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none' x:num="202.614">202.614</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="203.78200000000001">203.782</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none' x:num="202.702">202.702</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none' x:num>200.39</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none' x:num="202.035">202.035</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="204.52500000000001">204.525</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="206.51599999999999">206.516</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="206.35499999999999">206.355</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="206.74100000000001">206.741</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none' x:num="179.988">179.988</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none' x:num="210.315">210.315</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none' x:num="205.464">205.464</td>
 
−
</tr>
 
−
<tr height=19 style='height:14.25pt'>
 
−
  <td height=19 class=xl47 style='height:14.25pt;border-top:none' x:num>45</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="13.385999999999999">13.386</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="214.14500000000001">214.145</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none' x:num="213.602">213.602</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none' x:num="219.416">219.416</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="223.43700000000001">223.437</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none' x:num="241.267">241.267</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="224.90899999999999">224.909</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="244.25700000000001">244.257</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="235.42699999999999">235.427</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none' x:num="243.904">243.904</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="224.78299999999999">224.783</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="235.97900000000001">235.979</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="239.01400000000001">239.014</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="209.00899999999999">209.009</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="214.66900000000001">214.669</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none' x:num>224.21</td>
 
−
</tr>
 
−
<tr height=19 style='height:14.25pt'>
 
−
  <td height=19 class=xl47 style='height:14.25pt;border-top:none' x:num>90</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none' x:num="11.541">11.541</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="273.66399999999999">273.664</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="245.62899999999999">245.629</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="250.21100000000001">250.211</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none' x:num="305.733">305.733</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="317.54700000000003">317.547</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none' x:num="313.495">313.495</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="299.86900000000003">299.869</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="324.39600000000002">324.396</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="328.15699999999998">328.157</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="331.19400000000002">331.194</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="309.16699999999997">309.167</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none' x:num>305.24</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="261.80099999999999">261.801</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="267.36900000000003">267.369</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
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  x:num="281.32799999999997">281.328</td>
 
−
</tr>
 
−
<tr height=19 style='height:14.25pt'>
 
−
  <td height=19 class=xl47 style='height:14.25pt;border-top:none' x:num>135</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none' x:num="11.894">11.894</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="344.98700000000002">344.987</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="319.85399999999998">319.854</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="325.42099999999999">325.421</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="370.16699999999997">370.167</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="398.35199999999998">398.352</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="369.30900000000003">369.309</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="372.48700000000002">372.487</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="407.47800000000001">407.478</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="385.07299999999998">385.073</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="333.98899999999998">333.989</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none' x:num>355.2</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="351.92899999999997">351.929</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="314.24599999999998">314.246</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none' x:num>332.2</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="351.36399999999998">351.364</td>
 
−
</tr>
 
−
<tr height=19 style='height:14.25pt'>
 
−
  <td height=19 class=xl47 style='height:14.25pt;border-top:none' x:num>180</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="11.066000000000001">11.066</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="424.18200000000002">424.182</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="425.56400000000002">425.564</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="389.78500000000003">389.785</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="415.84399999999999">415.844</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="463.18400000000003">463.184</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="378.60599999999999">378.606</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="447.50799999999998">447.508</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none' x:num>456.27</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="449.41399999999999">449.414</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none' x:num="311.226">311.226</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="441.90199999999999">441.902</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="456.96899999999999">456.969</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none' x:num="338.101">338.101</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none'
 
−
  x:num="304.14600000000002">304.146</td>
 
−
  <td class=xl47 style='border-top:none;border-left:none' x:num="349.339">349.339</td>
 
−
</tr>
 
−
<![if supportMisalignedColumns]>
 
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  <td width=72 style='width:54pt'></td>
 
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  <td width=72 style='width:54pt'></td>
 
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  <td width=72 style='width:54pt'></td>
 
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  <td width=72 style='width:54pt'></td>
 
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  <td width=72 style='width:54pt'></td>
 
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</tr>
 
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<![endif]>
 
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</table>
 
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<!-- Uncomment this to enable Functional Parameter display
 
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===Functional Parameters===
 
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<partinfo>BBa_K741001 parameters</partinfo>
 
−
<!-- -->
 

Latest revision as of 20:07, 2 October 2012

plac-RBS-cI-T

Lac promoter with cI gene downstream. LacI or glucose can repress the expression of cI while lactose and IPTG can activates it.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]