Difference between revisions of "Part:BBa K741001"

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<partinfo>BBa_K741001 short</partinfo>
 
<partinfo>BBa_K741001 short</partinfo>
  
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It can generates protein CI whereas the expression can only be started when the promoter plac is activated by IPTG.
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Lac promoter with cI gene downstream. LacI or glucose can repress the expression of cI while lactose and IPTG can activates it.
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K741001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K741001 SequenceAndFeatures</partinfo>
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== Data ==
 
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<p> Fluorescence intensity </p>
 
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{| {{table}}
 
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{|class="sortable" style="border:3px solid black; width: 70%" align="center"
 
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| align="center" style="background:#f0f0f0;"|'''t/min'''
 
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| align="center" style="background:#f0f0f0;"|'''TM9 medium'''
 
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| align="center" style="background:#f0f0f0;"|'''0'''
 
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| align="center" style="background:#f0f0f0;"|'''500mM glucose'''
 
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| align="center" style="background:#f0f0f0;"|'''800mM glucose'''
 
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| align="center" style="background:#f0f0f0;"|'''1000mM glucose'''
 
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| align="center" style="background:#f0f0f0;"|'''1000mM IPTG'''
 
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0 11.801 197.047 198.876 190.953 202.614 203.782 202.702 200.39 202.035 204.525 206.516 206.355 206.741 179.988 210.315 205.464
 
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45 13.386 214.145 213.602 219.416 223.437 241.267 224.909 244.257 235.427 243.904 224.783 235.979 239.014 209.009 214.669 224.21
 
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90 11.541 273.664 245.629 250.211 305.733 317.547 313.495 299.869 324.396 328.157 331.194 309.167 305.24 261.801 267.369 281.328
 
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135 11.894 344.987 319.854 325.421 370.167 398.352 369.309 372.487 407.478 385.073 333.989 355.2 351.929 314.246 332.2 351.364
 
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180 11.066 424.182 425.564 389.785 415.844 463.184 378.606 447.508 456.27 449.414 311.226 441.902 456.969 338.101 304.146 349.339
 
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===Functional Parameters===
 
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<partinfo>BBa_K741001 parameters</partinfo>
 
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Latest revision as of 20:07, 2 October 2012

plac-RBS-cI-T

Lac promoter with cI gene downstream. LacI or glucose can repress the expression of cI while lactose and IPTG can activates it.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]