Difference between revisions of "Part:BBa K901012"

Latest revision as of 19:18, 2 October 2012

AatA Expression Construct

AatA (BBa_K901009) under control of the LacI promoter (BBa_J04500) with double terminator

Part of a 3 enzyme system designed to enhance the production of acetic acid by E. coli in the presence of high levels of sugars. ACK (BBa_K901015) and PTA (BBa_K901019) are enzymes that allow for the metabolism of acetic acid when there are low levels of available glucose. When glucose in the environment is high, these enzymes work in reverse as an overflow pathway for central metabolism, converting excess acetyl-CoA to acetate.

AatA is an ABC transporter found in Acetobacter acetii which moves acetate out of the cell. This enzyme is introduced to drive the production of acetate forward and to confer resistance to high levels of acetic acid in the cellular environment.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K901012 short</partinfo>
-pLacI regulate AatA with double terminator
+AatA ([https://parts.igem.org/Part:BBa_K901009 BBa_K901009]) under control of the LacI promoter ([https://parts.igem.org/Part:BBa_J04500 BBa_J04500]) with double terminator
+Part of a 3 enzyme system designed to enhance the production of acetic acid by <i>E. coli</i> in the presence of high levels of sugars. ACK ([https://parts.igem.org/Part:BBa_K901015 BBa_K901015]) and PTA ([https://parts.igem.org/Part:BBa_K901019 BBa_K901019]) are enzymes that allow for the metabolism of acetic acid when there are low levels of available glucose. When glucose in the environment is high, these enzymes work in reverse as an overflow pathway for central metabolism, converting excess acetyl-CoA to acetate.
+AatA is an ABC transporter found in <i>Acetobacter acetii</i> which moves acetate out of the cell. This enzyme is introduced to drive the production of acetate forward and to confer resistance to high levels of acetic acid in the cellular environment.
 <!-- Add more about the biology of this part here