Difference between revisions of "Part:BBa K741001:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
−
TBD
+
We think it improved BBa_P0451(RBS-cI-Terminator) in parts.igem.
−
It can generates protein CI whereas the expression can only be started when the promoter plac is activated by IPTG.
+
 
 +
However, we think the lac promoter cannot work well on pSB1C3 for there is not lacI gene on it. And K12 strains can not express enough lacI to repress the expression of plac. So the leak expression of lac promoter is quite high. We use glucose in our later experiments to repress plac. But the results are not that good.
  
 
===Source===
 
===Source===

Revision as of 19:13, 2 October 2012

plac-RBS-cI-T


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We think it improved BBa_P0451(RBS-cI-Terminator) in parts.igem.

However, we think the lac promoter cannot work well on pSB1C3 for there is not lacI gene on it. And K12 strains can not express enough lacI to repress the expression of plac. So the leak expression of lac promoter is quite high. We use glucose in our later experiments to repress plac. But the results are not that good.

Source

BBa_R0010 BBa_P0451

References