Difference between revisions of "Part:BBa K741000:Design"

 
(→‎Source)
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K741000 short</partinfo>
 
<partinfo>BBa_K741000 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
You need a promoter and a terminator but no RBS.
+
We construct this part by PCR with design primers which can reversely arrange the sites for EcoRI, XbaI, SpeI, PstI. The template DNA we use is the genome of lambda phage.
−
 
+
  
 +
You need a promoter and a terminator but no RBS when using this part.
  
 
===Source===
 
===Source===
  
−
BBa_I759002
+
Genome of lambda phage
  
 
===References===
 
===References===

Latest revision as of 19:01, 2 October 2012

anticro


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 134
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We construct this part by PCR with design primers which can reversely arrange the sites for EcoRI, XbaI, SpeI, PstI. The template DNA we use is the genome of lambda phage.

You need a promoter and a terminator but no RBS when using this part.

Source

Genome of lambda phage

References