Difference between revisions of "Part:BBa K901003"
 
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Coding sequence for Invertase A ([https://parts.igem.org/Part:BBa_K901000 BBa_K901000]) under the control of a LacI promoter with a sRBS ([https://parts.igem.org/Part:BBa_J04500 BBa_J04500]).
 
Coding sequence for Invertase A ([https://parts.igem.org/Part:BBa_K901000 BBa_K901000]) under the control of a LacI promoter with a sRBS ([https://parts.igem.org/Part:BBa_J04500 BBa_J04500]).
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This expression construct is part of a 3 enzyme system designed to enhance the production and secretion of monosaccharide sugars in <i>Synechococcus elongatus</i>. This system is comprised of InvA, Galu ([https://parts.igem.org/Part:BBa_K901007 BBa_K901007]), and Glf ([https://parts.igem.org/Part:BBa_K901008 BBa_K901008]).
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GalU codes for UDP-glucose phosphorylase. This is an enzyme from <i>E. coli</i> that produces UDP-glucose. The presence of UDP-glucose enhances the native production of the disaccharide sucrose in <i>S. elongatus</i> in response to salt shock.
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Sucrose is then cleaved into glucose and fructose by Invertase A and secreted from the cell via the membrane bound transport protein Glf. These monosaccharides are sufficient nutriment for <i>E. coli</i> and will be used to feed a culture of engineered cells designed to produce high levels of acetic acid.
  
 
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Latest revision as of 18:57, 2 October 2012

InvA Expression Construct

Coding sequence for Invertase A (BBa_K901000) under the control of a LacI promoter with a sRBS (BBa_J04500).

This expression construct is part of a 3 enzyme system designed to enhance the production and secretion of monosaccharide sugars in Synechococcus elongatus. This system is comprised of InvA, Galu (BBa_K901007), and Glf (BBa_K901008).

GalU codes for UDP-glucose phosphorylase. This is an enzyme from E. coli that produces UDP-glucose. The presence of UDP-glucose enhances the native production of the disaccharide sucrose in S. elongatus in response to salt shock.

Sucrose is then cleaved into glucose and fructose by Invertase A and secreted from the cell via the membrane bound transport protein Glf. These monosaccharides are sufficient nutriment for E. coli and will be used to feed a culture of engineered cells designed to produce high levels of acetic acid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 287
    Illegal NgoMIV site found at 1199
  • 1000
    COMPATIBLE WITH RFC[1000]