Difference between revisions of "Part:BBa K887011"
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The following figure demonstrate specific DNA sequence those three zinc finger functional domains can recognize | The following figure demonstrate specific DNA sequence those three zinc finger functional domains can recognize | ||
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If we design a DNA sequence which can make these fusion proteins stay in order, just like a production line, we can achieve our goal, rising yield of isobutanol production because of better efficiency of reaction.<br> | If we design a DNA sequence which can make these fusion proteins stay in order, just like a production line, we can achieve our goal, rising yield of isobutanol production because of better efficiency of reaction.<br> | ||
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However, after sequencing, we found that there is a deletion of a base pair in the zif268 biding site | However, after sequencing, we found that there is a deletion of a base pair in the zif268 biding site | ||
| − | [[image:DDpicture1.jpg| | + | [[image:DDpicture1.jpg|700px]]<br> |
Revision as of 15:06, 2 October 2012
DNA program : specific DNA sequence which can be recognized by zinc fingers
In order to promote the yield of isobutanol further, we not only use the temperature control system we designed but also encode three different kinds of zinc finger functional domain genes (zif268 : Part:BBa_K323105, PBSll : Part:BBa_K323107 and hivC : Part:BBa_K165006) preceding each enzyme genes.(More details about the biobrick, please refer to Part:BBa_K887000)
It means that these fusion proteins (combination of zinc finger functional domain and our enzymes) will bind specific DNA sequence and also carry out isobutanol biosynthesis reaction simultaneously.
The following figure demonstrate specific DNA sequence those three zinc finger functional domains can recognize
If we design a DNA sequence which can make these fusion proteins stay in order, just like a production line, we can achieve our goal, rising yield of isobutanol production because of better efficiency of reaction.
Fortunately, the design of our biobricks has the same order of zinc finger as 2010 Slovenia iGEM team, so we decided to use their DNA program (Part:BBa_K323066) instead of synthesizing one.
However, after sequencing, we found that there is a deletion of a base pair in the zif268 biding site
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]