Difference between revisions of "Part:BBa K887011"

Revision as of 14:55, 2 October 2012

DNA program : specific DNA sequence which can be recognized by zinc fingers

In order to promote the yield of isobutanol further, we not only use the temperature control system we designed but also encode three different kinds of zinc finger functional domain genes (zif268 : Part:BBa_K323105, PBSll : Part:BBa_K323107 and hivC : Part:BBa_K165006) preceding each enzyme genes.(More details about the biobrick, please refer to Part:BBa_K887000)

It means that these fusion proteins (combination of zinc finger functional domain and our enzymes) will bind specific DNA sequence and also carry out isobutanol biosynthesis reaction simultaneously.

The following figure demonstrate specific DNA sequence those three zinc finger functional domains can recognize

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 In order to promote the yield of isobutanol further, we not only use the temperature control system we designed but also encode three different kinds of zinc finger functional domain genes (zif268 : [[Part:BBa_K323105]], PBSll : [[Part:BBa_K323107]] and hivC : [[Part:BBa_K165006]]) preceding each enzyme genes.(More details about the biobrick, please refer to [[Part:BBa_K887000]])<br>
-It means that these fusion proteins (combination of zinc finger functional domain and our enzymes) will bind specific DNA sequence and also carry out isobutanol biosynthesis reaction simultaneously.
+It means that these fusion proteins (combination of zinc finger functional domain and our enzymes) will bind specific DNA sequence and also carry out isobutanol biosynthesis reaction simultaneously.<br>
+The following figure demonstrate specific DNA sequence those three zinc finger functional domains can recognize