Difference between revisions of "Part:BBa K799003"
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===Plasmid Map=== | ===Plasmid Map=== | ||
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===Sequencing=== | ===Sequencing=== | ||
Revision as of 04:54, 2 October 2012
Ethanol/Stress Inducible YJL052W Promoter
____ promoter shows an increase in protein expression when exposed to __% ethanol.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 171
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization
This is fluorescence data for the expression of GFP under the control of YJL200C, our ethanol induced promoter. Fluorescence can be seen soon after ethanol induction. Furthermore, the fluorescence plateaus but remains significant till our last time point of 230 minutes.
Plasmid Map
Sequencing
This is the sequencing result for promoter YJL052W. As can be seen, it is a perfect match to the genomic DNA promoter.