Difference between revisions of "Part:BBa K799009"

Revision as of 21:22, 1 October 2012

Ethanol/Stress Inducible YMR251W-A Promoter

YMR251W-A promoter shows an increase in protein expression when exposed to up to 14% ethanol.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 120
Illegal PstI site found at 277
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 169
Illegal NheI site found at 355
Illegal PstI site found at 277
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 120
Illegal PstI site found at 277
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 120
Illegal PstI site found at 277
1000
COMPATIBLE WITH RFC[1000]

Characterization

This is fluorescence data for the expression of GFP under the control of YMR251W-A, our ethanol induced promoter. Fluorescence can be seen soon after ethanol induction. Furthermore, the fluorescence plateaus but remains significant till our last time point of 230 minutes.

Plasmid Map

[[]]

Sequencing

This is the sequencing result for YMR251W-A. As can be seen, it is a perfect match to the genomic DNA promoter.

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K799009 short</partinfo>
-____ promoter shows an increase in protein expression when exposed to __% ethanol.
+YMR251W-A promoter shows an increase in protein expression when exposed to up to 14% ethanol.
 <!-- Add more about the biology of this part here
@@ Line 11: / Line 11: @@
 <partinfo>BBa_K799009 SequenceAndFeatures</partinfo>
+===Characterization===
+[[Image:ProA5 fluoro.png]]
+This is fluorescence data for the expression of GFP under the control of YMR251W-A, our ethanol induced promoter. Fluorescence can be seen soon after ethanol induction. Furthermore, the fluorescence plateaus but remains significant till our last time point of 230 minutes.
 ===Plasmid Map===