Difference between revisions of "Part:BBa K799007"

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<partinfo>BBa_K799007 short</partinfo>
 
<partinfo>BBa_K799007 short</partinfo>
  
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____ promoter shows an increase in protein expression when exposed to __% ethanol.
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YMR170C promoter shows an increase in protein expression when exposed to up to 14% ethanol.
  
 
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<partinfo>BBa_K799007 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K799007 SequenceAndFeatures</partinfo>
  
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===Characterization===
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[[Image:ProA4 fluoro.png]]
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This is fluorescence data for the expression of GFP under the control of YMR170C, our ethanol induced promoter. Fluorescence can be seen soon after ethanol induction. Furthermore, the fluorescence plateaus but remains significant till our last time point of 230 minutes.
  
 
===Plasmid Map===
 
===Plasmid Map===

Revision as of 21:19, 1 October 2012

Ethanol/Stress Inducible YMR170C Promoter

YMR170C promoter shows an increase in protein expression when exposed to up to 14% ethanol.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

ProA4 fluoro.png

This is fluorescence data for the expression of GFP under the control of YMR170C, our ethanol induced promoter. Fluorescence can be seen soon after ethanol induction. Furthermore, the fluorescence plateaus but remains significant till our last time point of 230 minutes.

Plasmid Map

[[]]


Sequencing

ProA4 sequencing.png

This is the sequencing result for YMR170C promoter. As can be seen, it is a perfect match to the genomic DNA promoter.