Difference between revisions of "Part:BBa K917005:Design"

 
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<partinfo>BBa_K917005 short</partinfo>
 
<partinfo>BBa_K917005 short</partinfo>
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===Design Notes===
 
===Design Notes===
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This part includes the native ribosome binding site for nitroreductase.
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This part includes the native ribosome binding site. The original sequence (http://www.ncbi.nlm.nih.gov/nuccore/M63808.1) had a PstI restriction site however the cloned gene did not have PstI restriction site ( confirmed trough sequencing and restriction digest).
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===Source===
 
===Source===

Revision as of 20:32, 1 October 2012

lac promoter plus nitroreductase (nsfI) gene from Enterobacter cloacae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1189
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part includes the native ribosome binding site. The original sequence (http://www.ncbi.nlm.nih.gov/nuccore/M63808.1) had a PstI restriction site however the cloned gene did not have PstI restriction site ( confirmed trough sequencing and restriction digest).

Source

Enterobacter cloacae

References