Difference between revisions of "Part:BBa K929100"
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| − | The single chain fragment (scFV425) specifically binds to the epidermal growth factor receptor (EGFR) domain 3. The part has a shortened N-terminal FLAG-tag sequence of five amino acids (DYKDE) for purification and detection.<br> | + | The single chain fragment (scFV425) specifically binds to the epidermal growth factor receptor (EGFR) domain 3. The part has a shortened N-terminal FLAG-tag sequence of five amino acids (DYKDE) for purification and detection. For characterisation and further applications see BBa_K9291001.<br> |
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Revision as of 17:55, 1 October 2012
Anti-EGFR scFv425
General information
| Anti-EGFR scFv425 | |
|---|---|
| |
| BioBrick Nr. | BBa_929100 |
| RFC standard | RFC 25 |
| Requirement | pSB1C3 |
| Source | plasmid pKMEF425bla |
| Submitted by | [http://2012.igem.org/Team:Potsdam_Bioware Potsdam_Bioware2012] |
The single chain fragment (scFV425) specifically binds to the epidermal growth factor receptor (EGFR) domain 3. The part has a shortened N-terminal FLAG-tag sequence of five amino acids (DYKDE) for purification and detection. For characterisation and further applications see BBa_K9291001.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 538
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]