Difference between revisions of "Part:BBa K864150"
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<partinfo>BBa_K864150 short</partinfo> | <partinfo>BBa_K864150 short</partinfo> | ||
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Consists of chloramphenicol resistance marker [[Part:BBa_P1004|BBa_P1004]] and the Bacillus subtilis levansucrase SacB [[Part:BBa_K322921|BBa_K322921]] | Consists of chloramphenicol resistance marker [[Part:BBa_P1004|BBa_P1004]] and the Bacillus subtilis levansucrase SacB [[Part:BBa_K322921|BBa_K322921]] | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | [[Image:Cat-SacB.png | 600px]] <br> | ||
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| + | '''iGEM12_Uppsala_University:''' ''E. coli strain carrying a chromosomally integrated copy of cat-sacB (<partinfo>BBa_K864150</partinfo>) plated on a sucrose plate (A) and on a chloramphenicol plate (B). E. coli wildtype strain MG1655 was used as a control.'' | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K864150 parameters</partinfo> | <partinfo>BBa_K864150 parameters</partinfo> | ||
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Latest revision as of 20:32, 30 September 2012
Cat-SacB cassette for scarless chromosomal knockout
Cat-SacB
Consists of chloramphenicol resistance marker BBa_P1004 and the Bacillus subtilis levansucrase SacB BBa_K322921
Usage and Biology
iGEM12_Uppsala_University: E. coli strain carrying a chromosomally integrated copy of cat-sacB (BBa_K864150) plated on a sucrose plate (A) and on a chloramphenicol plate (B). E. coli wildtype strain MG1655 was used as a control.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]