Difference between revisions of "Part:BBa K845005"

Latest revision as of 17:09, 30 September 2012

TapZ engineered membrane receptor

TapZ is an engineered membrane receptor capable of detecting several didpeptides.

Once activated TapZ phosphorylates the ompR protein which is then an active transcription factor.

Usage and Biology

In order to detect the S.Aureus we take inspiration on the parasite project ( http://2010.igem.org/Team:Imperial_College_London ) but there was one problem there is no peptide detector in E.coli who was able to activate a promoter. One receptor, involved in chemotaxis, detects few di/tri peptides (Tap receptor). In order to activate a promoter in presence of that peptide we designed a hybrid Tap/EnvZ receptor (which we called TapZ). EnvZ is a receptor involved in osmotic pressure sensing. It phosphorylates ompR which activates the pompC promoter. This part must be used in a ΔenvZ strain.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K845005 short</partinfo>
-...
+TapZ is an engineered membrane receptor capable of detecting several didpeptides.
+Once activated TapZ phosphorylates the ompR protein which is then an active transcription factor.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+In order to detect the S.Aureus we take inspiration on the parasite project
+( http://2010.igem.org/Team:Imperial_College_London ) but there was one problem there is no peptide detector in E.coli who was able to activate a promoter. One receptor, involved in chemotaxis, detects few di/tri peptides (Tap receptor). In order to activate a promoter in presence of that peptide we designed a hybrid Tap/EnvZ receptor (which we called TapZ).
+EnvZ is a receptor involved in osmotic pressure sensing. It phosphorylates ompR which activates the pompC promoter.
+This part must be used in a <i>ΔenvZ</i> strain.
 <!-- -->