Difference between revisions of "Part:BBa K812110"
 
(3 intermediate revisions by 2 users not shown)
Line 2: Line 2:
 
<partinfo>BBa_K812110 short</partinfo>
 
<partinfo>BBa_K812110 short</partinfo>
  
−
This part is a super folded GFP fused to an AID tag for its recignition by the ubiquitinase E3 that induces its degradation in the presence of auxin in the eukaryote cell. his part is in pCS2+, the plasmid backbone is <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a></html>.
+
<html>
 +
</br>
 +
<b>*** WARNING : THIS PART HAS BEEN MERGED WITH <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812010">BBa_K812010</a>. IT IS AVAILABLE IN pSB1C3 AND IN FROG-READY PLASMID BACKBONES <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a>.***</b>
 +
</br></br>
 +
 
 +
This part is a super folded GFP fused to an AID tag for its recignition by the ubiquitinase E3 that induces its degradation in the presence of auxin in the eukaryote cell. his part is in pCS2+, the plasmid backbone is <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a></html> and the device is from  <html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812010">BBa_K812010</a></html>.
  
 
This part is a part of a device pattented by Kanemaki Masato, Kakimoto Tatsuo, Nishimura Kohei, Takisawa Haruhiko and Fukagawa Tatsuo for yeast and mammalian cells use (http://www.freepatentsonline.com/y2012/0115232.html). However the pattent does not cover the use for oviparian such as frogs and chicken.
 
This part is a part of a device pattented by Kanemaki Masato, Kakimoto Tatsuo, Nishimura Kohei, Takisawa Haruhiko and Fukagawa Tatsuo for yeast and mammalian cells use (http://www.freepatentsonline.com/y2012/0115232.html). However the pattent does not cover the use for oviparian such as frogs and chicken.
  
 
The GFP-AID contains 2 NLS domains that relocate the protein in the nucleus of the cells for a better signal concentration.
 
The GFP-AID contains 2 NLS domains that relocate the protein in the nucleus of the cells for a better signal concentration.
−
<html><br>
+
<br>
 
<img src="https://static.igem.org/mediawiki/parts/c/ce/Picture_genethon_GFP-aid_7.09.jpg" alt="perdu" width="880px" /> <br/><br/>
 
<img src="https://static.igem.org/mediawiki/parts/c/ce/Picture_genethon_GFP-aid_7.09.jpg" alt="perdu" width="880px" /> <br/><br/>
 
</html>
 
</html>

Latest revision as of 17:01, 30 September 2012

GFP fusion with the ubuquitinase E3 OsTirI recoginition domain for PSC2+ plasmid


*** WARNING : THIS PART HAS BEEN MERGED WITH BBa_K812010. IT IS AVAILABLE IN pSB1C3 AND IN FROG-READY PLASMID BACKBONES BBa_K812000.***

This part is a super folded GFP fused to an AID tag for its recignition by the ubiquitinase E3 that induces its degradation in the presence of auxin in the eukaryote cell. his part is in pCS2+, the plasmid backbone is BBa_K812000 and the device is from BBa_K812010.

This part is a part of a device pattented by Kanemaki Masato, Kakimoto Tatsuo, Nishimura Kohei, Takisawa Haruhiko and Fukagawa Tatsuo for yeast and mammalian cells use (http://www.freepatentsonline.com/y2012/0115232.html). However the pattent does not cover the use for oviparian such as frogs and chicken.

The GFP-AID contains 2 NLS domains that relocate the protein in the nucleus of the cells for a better signal concentration.
<img src="Picture_genethon_GFP-aid_7.09.jpg" alt="perdu" width="880px" />

</html> The tadpole 1 express GFP-aid in epidermic cells, whereas the tadpole 2 express GFP-aid in optical nerve,nostril nerve, tail muscle cells and branchial basket.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1020
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1405