Difference between revisions of "Part:BBa K812120"

 
 
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__NOTOC__
 
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<partinfo>BBa_K812120 short</partinfo>
 
<partinfo>BBa_K812120 short</partinfo>
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<b>*** WARNING : THIS PART HAS BEEN MERGED WITH <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812020">BBa_K812020</a>. IT IS AVAILABLE IN pSB1C3 AND IN FROG-READY PLASMID BACKBONES <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a>.***</b>
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Corresponds to :
 
Corresponds to :
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Eukaryotic PoPs -> IaaH. Indoleacetamide hydrolyase for Auxin generator, with kozak, which you can find biobricked here on the plasmid pSB1C3: BBa_K812020.
 
  
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on the plasmid PCS2+ described here: BBa_K812000
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This part contains the coding sequence for Indoleacetamide hydrolyase (IaaH). This part is from  <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K515100">BBa_K515100</a>, but it was modified to increase the expression in eukaryotic cells by adding a Kozak sequence in front of the original sequence.</br></br>
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This part have the plasmid backbone <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K8120000</a> and the part J04450 is replaced by IaaH <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812020">BBa_K812000</a>.</br></br>
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This plasmid is ready for injection in tadpole.</br>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 16:58, 30 September 2012

IaaH on PSC2+ Ready for use in xenopus tropicalis egg


*** WARNING : THIS PART HAS BEEN MERGED WITH BBa_K812020. IT IS AVAILABLE IN pSB1C3 AND IN FROG-READY PLASMID BACKBONES BBa_K812000.***

Corresponds to : This part contains the coding sequence for Indoleacetamide hydrolyase (IaaH). This part is from BBa_K515100, but it was modified to increase the expression in eukaryotic cells by adding a Kozak sequence in front of the original sequence.

This part have the plasmid backbone BBa_K8120000 and the part J04450 is replaced by IaaH BBa_K812000.

This plasmid is ready for injection in tadpole.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1025
  • 1000
    COMPATIBLE WITH RFC[1000]