Difference between revisions of "Part:BBa K812132"

 
(One intermediate revision by the same user not shown)
Line 2: Line 2:
 
<partinfo>BBa_K812132 short</partinfo>
 
<partinfo>BBa_K812132 short</partinfo>
  
mCFP fused with a kozak sequence to enhance its expression in eukaryotic cells. To be used with an eukaryotic expression system such as pSC2+ <html>(<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">K812000</a>)</html> and related parts.
+
 
 
<html>
 
<html>
 +
</br>
 +
<b>*** WARNING : THIS PART HAS BEEN MERGED WITH <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812032">BBa_K812032</a>. IT IS AVAILABLE IN pSB1C3 AND IN FROG-READY PLASMID BACKBONES <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">BBa_K812000</a>.***</b>
 +
</br></br>
 +
 +
mCFP fused with a kozak sequence to enhance its expression in eukaryotic cells. To be used with an eukaryotic expression system such as pSC2+ <html>(<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K812000">K812000</a>)</html> and related parts.
 +
 
<br>
 
<br>
 
<img src="https://static.igem.org/mediawiki/2012/5/58/CFP_tadpole.jpg" alt="perdu" width="880px" />
 
<img src="https://static.igem.org/mediawiki/2012/5/58/CFP_tadpole.jpg" alt="perdu" width="880px" />

Latest revision as of 16:56, 30 September 2012

mCFP with kozak sequence for expression in Xenopus



*** WARNING : THIS PART HAS BEEN MERGED WITH BBa_K812032. IT IS AVAILABLE IN pSB1C3 AND IN FROG-READY PLASMID BACKBONES BBa_K812000.***

mCFP fused with a kozak sequence to enhance its expression in eukaryotic cells. To be used with an eukaryotic expression system such as pSC2+ (K812000) and related parts.


<img src="CFP_tadpole.jpg" alt="perdu" width="880px" />
</html> Tadpole 1 is 2 days (stage 36-38), the expression of CFP is localized in intestine. Tadpole 2 is 4 days (stage 45-46), the expression of CFP is localized in Branchial basket and intestine

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]