Difference between revisions of "Part:BBa K741009"
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[[Image:USTC2012Result5_副本.png]] | [[Image:USTC2012Result5_副本.png]] | ||
<p>Figure 5 shows the difference in intensity of expression of pCon(0.244) and pCon(0.856). Obviously pCon(0.856) expresses much stronger than pCon(0.244).</p> | <p>Figure 5 shows the difference in intensity of expression of pCon(0.244) and pCon(0.856). Obviously pCon(0.856) expresses much stronger than pCon(0.244).</p> | ||
+ | |||
+ | ==Data== | ||
+ | |||
+ | <table x:str border=0 cellpadding=0 cellspacing=0 width=683 style='border-collapse: | ||
+ | collapse;table-layout:fixed;width:512pt'> | ||
+ | <col width=72 span=6 style='width:54pt'> | ||
+ | <col width=179 style='mso-width-source:userset;mso-width-alt:5728;width:134pt'> | ||
+ | <col width=72 style='width:54pt'> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td colspan=8 height=19 class=xl26 width=683 style='height:14.25pt; | ||
+ | width:512pt'>pCon(0.856)-RBS-crogfp-T</td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl27 style='height:14.25pt'> </td> | ||
+ | <td colspan=3 class=xl27>OD</td> | ||
+ | <td class=xl28> </td> | ||
+ | <td colspan=3 class=xl29>FLU</td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl27 style='height:14.25pt'>t/min</td> | ||
+ | <td class=xl28>Parallel</td> | ||
+ | <td class=xl28>Experime<span style='display:none'>ntal group</span></td> | ||
+ | <td class=xl28 colspan=2 style='mso-ignore:colspan'>M9 medium</td> | ||
+ | <td class=xl28>Parallel</td> | ||
+ | <td class=xl28>Experimental group</td> | ||
+ | <td class=xl28>M9 medium</td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl30 style='height:14.25pt' x:num>0</td> | ||
+ | <td class=xl30 x:num>1</td> | ||
+ | <td class=xl31 align=right x:num="6.8000000000000005E-2">0.068</td> | ||
+ | <td colspan=2 class=xl31 style='mso-ignore:colspan'></td> | ||
+ | <td class=xl30 x:num>1</td> | ||
+ | <td class=xl31 align=right x:num="611.19">611.19</td> | ||
+ | <td class=xl31></td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl30 style='height:14.25pt'></td> | ||
+ | <td class=xl30 x:num>2</td> | ||
+ | <td class=xl31 align=right x:num="6.9599999999999995E-2">0.0696</td> | ||
+ | <td colspan=2 class=xl31 style='mso-ignore:colspan'></td> | ||
+ | <td class=xl30 x:num>2</td> | ||
+ | <td class=xl31 align=right x:num="560.005">560.005</td> | ||
+ | <td class=xl31></td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl30 style='height:14.25pt'></td> | ||
+ | <td class=xl30 x:num>3</td> | ||
+ | <td class=xl31 align=right x:num="7.4099999999999999E-2">0.0741</td> | ||
+ | <td class=xl31 align=right x:num="4.1599999999999998E-2">0.0416</td> | ||
+ | <td class=xl31></td> | ||
+ | <td class=xl30 x:num>3</td> | ||
+ | <td class=xl31 align=right x:num="540.94899999999996">540.949</td> | ||
+ | <td class=xl31 align=right x:num="14.037000000000001">14.037</td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl30 style='height:14.25pt' x:num>45</td> | ||
+ | <td class=xl30 x:num>1</td> | ||
+ | <td class=xl31 align=right x:num="7.8700000000000006E-2">0.0787</td> | ||
+ | <td colspan=2 class=xl31 style='mso-ignore:colspan'></td> | ||
+ | <td class=xl30 x:num>1</td> | ||
+ | <td class=xl31 align=right x:num="625.16600000000005">625.166</td> | ||
+ | <td class=xl31></td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl30 style='height:14.25pt'></td> | ||
+ | <td class=xl30 x:num>2</td> | ||
+ | <td class=xl31 align=right x:num="7.5700000000000003E-2">0.0757</td> | ||
+ | <td colspan=2 class=xl31 style='mso-ignore:colspan'></td> | ||
+ | <td class=xl30 x:num>2</td> | ||
+ | <td class=xl31 align=right x:num="587.64700000000005">587.647</td> | ||
+ | <td class=xl31></td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl30 style='height:14.25pt'></td> | ||
+ | <td class=xl30 x:num>3</td> | ||
+ | <td class=xl31 align=right x:num="7.46E-2">0.0746</td> | ||
+ | <td class=xl31 align=right x:num="4.02E-2">0.0402</td> | ||
+ | <td class=xl31></td> | ||
+ | <td class=xl30 x:num>3</td> | ||
+ | <td class=xl31 align=right x:num="617.61199999999997">617.612</td> | ||
+ | <td class=xl31 align=right x:num="14.013">14.013</td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl30 style='height:14.25pt' x:num>90</td> | ||
+ | <td class=xl30 x:num>1</td> | ||
+ | <td class=xl31 align=right x:num="8.5800000000000001E-2">0.0858</td> | ||
+ | <td colspan=2 class=xl31 style='mso-ignore:colspan'></td> | ||
+ | <td class=xl30 x:num>1</td> | ||
+ | <td class=xl31 align=right x:num="712.78499999999997">712.785</td> | ||
+ | <td class=xl31></td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl30 style='height:14.25pt'></td> | ||
+ | <td class=xl30 x:num>2</td> | ||
+ | <td class=xl31 align=right x:num="8.2699999999999996E-2">0.0827</td> | ||
+ | <td colspan=2 class=xl31 style='mso-ignore:colspan'></td> | ||
+ | <td class=xl30 x:num>2</td> | ||
+ | <td class=xl31 align=right x:num="673.46500000000003">673.465</td> | ||
+ | <td class=xl31></td> | ||
+ | </tr> | ||
+ | <tr height=17 style='mso-height-source:userset;height:12.75pt'> | ||
+ | <td height=17 class=xl30 style='height:12.75pt'></td> | ||
+ | <td class=xl30 x:num>3</td> | ||
+ | <td class=xl31 align=right x:num="8.14E-2">0.0814</td> | ||
+ | <td class=xl31 align=right x:num="4.1099999999999998E-2">0.0411</td> | ||
+ | <td class=xl31></td> | ||
+ | <td class=xl30 x:num>3</td> | ||
+ | <td class=xl31 align=right x:num="648.529">648.529</td> | ||
+ | <td class=xl31 align=right x:num="14.449">14.449</td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl30 style='height:14.25pt' x:num>135</td> | ||
+ | <td class=xl30 x:num>1</td> | ||
+ | <td class=xl31 align=right x:num="9.3899999999999997E-2">0.0939</td> | ||
+ | <td colspan=2 class=xl31 style='mso-ignore:colspan'></td> | ||
+ | <td class=xl30 x:num>1</td> | ||
+ | <td class=xl31 align=right x:num="769.154">769.154</td> | ||
+ | <td class=xl31></td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl30 style='height:14.25pt'></td> | ||
+ | <td class=xl30 x:num>2</td> | ||
+ | <td class=xl31 align=right x:num="8.8700000000000001E-2">0.0887</td> | ||
+ | <td colspan=2 class=xl31 style='mso-ignore:colspan'></td> | ||
+ | <td class=xl30 x:num>2</td> | ||
+ | <td class=xl31 align=right x:num="740.30899999999997">740.309</td> | ||
+ | <td class=xl31></td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl30 style='height:14.25pt'></td> | ||
+ | <td class=xl30 x:num>3</td> | ||
+ | <td class=xl31 align=right x:num="8.77E-2">0.0877</td> | ||
+ | <td class=xl31 align=right x:num="4.19E-2">0.0419</td> | ||
+ | <td class=xl31></td> | ||
+ | <td class=xl30 x:num>3</td> | ||
+ | <td class=xl31 align=right x:num="747.65800000000002">747.658</td> | ||
+ | <td class=xl31 align=right x:num="13.826000000000001">13.826</td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl30 style='height:14.25pt' x:num>180</td> | ||
+ | <td class=xl30 x:num>1</td> | ||
+ | <td class=xl31 align=right x:num="0.1018">0.1018</td> | ||
+ | <td colspan=2 class=xl31 style='mso-ignore:colspan'></td> | ||
+ | <td class=xl30 x:num>1</td> | ||
+ | <td class=xl31 align=right x:num="800.94500000000005">800.945</td> | ||
+ | <td class=xl31></td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl30 style='height:14.25pt'></td> | ||
+ | <td class=xl30 x:num>2</td> | ||
+ | <td class=xl31 align=right x:num="9.1300000000000006E-2">0.0913</td> | ||
+ | <td colspan=2 class=xl31 style='mso-ignore:colspan'></td> | ||
+ | <td class=xl30 x:num>2</td> | ||
+ | <td class=xl31 align=right x:num="801.50800000000004">801.508</td> | ||
+ | <td class=xl31></td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl30 style='height:14.25pt'></td> | ||
+ | <td class=xl30 x:num>3</td> | ||
+ | <td class=xl31 align=right x:num="9.5299999999999996E-2">0.0953</td> | ||
+ | <td class=xl31 align=right x:num="3.9600000000000003E-2">0.0396</td> | ||
+ | <td class=xl31></td> | ||
+ | <td class=xl30 x:num>3</td> | ||
+ | <td class=xl31 align=right x:num="862.74400000000003">862.744</td> | ||
+ | <td class=xl31 align=right x:num="14.122999999999999">14.123</td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl30 style='height:14.25pt' x:num>225</td> | ||
+ | <td class=xl30 x:num>1</td> | ||
+ | <td class=xl31 align=right x:num="0.1182">0.1182</td> | ||
+ | <td colspan=2 class=xl31 style='mso-ignore:colspan'></td> | ||
+ | <td class=xl30 x:num>1</td> | ||
+ | <td class=xl31 align=right x:num="903.33900000000006">903.339</td> | ||
+ | <td class=xl31></td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl30 style='height:14.25pt'></td> | ||
+ | <td class=xl30 x:num>2</td> | ||
+ | <td class=xl31 align=right x:num="9.8900000000000002E-2">0.0989</td> | ||
+ | <td colspan=2 class=xl31 style='mso-ignore:colspan'></td> | ||
+ | <td class=xl30 x:num>2</td> | ||
+ | <td class=xl31 align=right x:num="936.18299999999999">936.183</td> | ||
+ | <td class=xl31></td> | ||
+ | </tr> | ||
+ | <tr height=19 style='height:14.25pt'> | ||
+ | <td height=19 class=xl30 style='height:14.25pt'></td> | ||
+ | <td class=xl30 x:num>3</td> | ||
+ | <td class=xl31 align=right x:num="0.1128">0.1128</td> | ||
+ | <td class=xl31 align=right x:num="4.7399999999999998E-2">0.0474</td> | ||
+ | <td class=xl31></td> | ||
+ | <td class=xl30 x:num>3</td> | ||
+ | <td class=xl31 align=right x:num="912.45600000000002">912.456</td> | ||
+ | <td class=xl31 align=right x:num="14.706">14.706</td> | ||
+ | </tr> | ||
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K741009 parameters</partinfo> | <partinfo>BBa_K741009 parameters</partinfo> | ||
<!-- --> | <!-- --> |
Revision as of 15:43, 30 September 2012
pCon(0.856)-RBS-crogfp-T
Here, pCon(J23108) constantly expressing the fusion protein crogfp at 50% of the strength of pCon(J23100).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 124
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 918
Figure 5 shows the difference in intensity of expression of pCon(0.244) and pCon(0.856). Obviously pCon(0.856) expresses much stronger than pCon(0.244).
Data
pCon(0.856)-RBS-crogfp-T | |||||||
OD | FLU | ||||||
t/min | Parallel | Experime | M9 medium | Parallel | Experimental group | M9 medium | |
0 | 1 | 0.068 | 1 | 611.19 | |||
2 | 0.0696 | 2 | 560.005 | ||||
3 | 0.0741 | 0.0416 | 3 | 540.949 | 14.037 | ||
45 | 1 | 0.0787 | 1 | 625.166 | |||
2 | 0.0757 | 2 | 587.647 | ||||
3 | 0.0746 | 0.0402 | 3 | 617.612 | 14.013 | ||
90 | 1 | 0.0858 | 1 | 712.785 | |||
2 | 0.0827 | 2 | 673.465 | ||||
3 | 0.0814 | 0.0411 | 3 | 648.529 | 14.449 | ||
135 | 1 | 0.0939 | 1 | 769.154 | |||
2 | 0.0887 | 2 | 740.309 | ||||
3 | 0.0877 | 0.0419 | 3 | 747.658 | 13.826 | ||
180 | 1 | 0.1018 | 1 | 800.945 | |||
2 | 0.0913 | 2 | 801.508 | ||||
3 | 0.0953 | 0.0396 | 3 | 862.744 | 14.123 | ||
225 | 1 | 0.1182 | 1 | 903.339 | |||
2 | 0.0989 | 2 | 936.183 | ||||
3 | 0.1128 | 0.0474 | 3 | 912.456 | 14.706 |