Difference between revisions of "Part:BBa K741001"

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Revision as of 15:20, 30 September 2012

plac-RBS-cI-T

It can generates protein CI whereas the expression can only be started when the promoter plac is activated by IPTG.

Sequence and Features

Data

Fluorescence intensity

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Fluorescence intensity
plac-RBS-GFP-T
t/min	M9 medium	0			500mM glucose			800mM glucose			1000mM glucose			1000mM IPTG
Parallel	1	1	2	3	1	2	3	1	2	3	1	2	3	1	2	3
0	11.801	197.047	198.876	190.953	202.614	203.782	202.702	200.39	202.035	204.525	206.516	206.355	206.741	179.988	210.315	205.464
45	13.386	214.145	213.602	219.416	223.437	241.267	224.909	244.257	235.427	243.904	224.783	235.979	239.014	209.009	214.669	224.21
90	11.541	273.664	245.629	250.211	305.733	317.547	313.495	299.869	324.396	328.157	331.194	309.167	305.24	261.801	267.369	281.328
135	11.894	344.987	319.854	325.421	370.167	398.352	369.309	372.487	407.478	385.073	333.989	355.2	351.929	314.246	332.2	351.364
180	11.066	424.182	425.564	389.785	415.844	463.184	378.606	447.508	456.27	449.414	311.226	441.902	456.969	338.101	304.146	349.339