Difference between revisions of "Part:BBa K845002"
Greghansen (Talk | contribs) |
Greghansen (Talk | contribs) |
||
| Line 4: | Line 4: | ||
RBS shine d'algarno | RBS shine d'algarno | ||
| − | GFP mut2 (S65A V68L S72A) | + | GFP mut2 (S65A V68L S72A): this variant used becomes fluorescent within less than five minutes of transcription initiation, and is highly stable and nonetoxic in <i>E. Coli</i>. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 13:20, 30 September 2012
Strong RBS GFPmut2
RBS shine d'algarno
GFP mut2 (S65A V68L S72A): this variant used becomes fluorescent within less than five minutes of transcription initiation, and is highly stable and nonetoxic in E. Coli.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 670