Difference between revisions of "Part:BBa K741012"

 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K741012 SequenceAndFeatures</partinfo>
 
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<p>To test the function of antisense RNA to cro (anticro) we have constructed, we measure the fluorescence intensity of plac-anticroT-pcon(0.856)-crogfp and plac-cI-prm-anticroT-pcon(0.856)-crogfp.</p>
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[[Image:USTC2012Result6_副本.png]]
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<p>At the beginning, the unit fluorescence intensity of the experimental groups with glucose added is the lowest, because glucose promotes the growth of E.coli., which makes the rate of fluorescence divided by OD lower than the other two groups.</p>
  
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<p>About 45 minutes later, the unit fluorescence intensity of experimental groups with glucose added is higher than the blank group, because glucose represses the expression of plac, which reduces the transcription of anticro. Therefore, the translation of crogfp is repressed less and the unit fluorescence intensity is higher.</p>
  
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<p>On the contrast, IPTG activates plac, which increases the transcription of anticro. Anticro binds to the mRNA crogfp and represses its translation.</p>
 
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===Functional Parameters===
 
===Functional Parameters===
 
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Revision as of 08:25, 30 September 2012

plac-anticro-T -pCon(0.856)-RBS-crogfp-T

The fusion protein crogfp is constantly expressed and the antisense RNA of cro is produced to inhibit the translation of the mRNA of crogfp after IPTG activated the promoter plac.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 124
    Illegal BglII site found at 1481
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 918

To test the function of antisense RNA to cro (anticro) we have constructed, we measure the fluorescence intensity of plac-anticroT-pcon(0.856)-crogfp and plac-cI-prm-anticroT-pcon(0.856)-crogfp.

USTC2012Result6 副本.png

At the beginning, the unit fluorescence intensity of the experimental groups with glucose added is the lowest, because glucose promotes the growth of E.coli., which makes the rate of fluorescence divided by OD lower than the other two groups.

About 45 minutes later, the unit fluorescence intensity of experimental groups with glucose added is higher than the blank group, because glucose represses the expression of plac, which reduces the transcription of anticro. Therefore, the translation of crogfp is repressed less and the unit fluorescence intensity is higher.

On the contrast, IPTG activates plac, which increases the transcription of anticro. Anticro binds to the mRNA crogfp and represses its translation.