Difference between revisions of "Part:BBa K864050:Design"

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===Source===
 
===Source===
  
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The pSC101ts origin was cloned from the pSIM5 plasmid from Datta et al, see reference below. One point mutation was made to remove one illegal SpeI site.
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The pSC101ts origin was cloned from the pSIM5 plasmid from Datta et al, see reference below.
  
 
===References===
 
===References===
 
[http://dx.doi.org/10.1016/j.gene.2006.04.018] Datta, S., Costantino, N. & Court, D.L. A set of recombineering plasmids for gram-negative bacteria. Gene 379, 109–115 (2006).
 
[http://dx.doi.org/10.1016/j.gene.2006.04.018] Datta, S., Costantino, N. & Court, D.L. A set of recombineering plasmids for gram-negative bacteria. Gene 379, 109–115 (2006).

Revision as of 23:51, 29 September 2012

pSC101 low copy origin of replication


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2130
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This ori was created by cloning pSC101ts from pSIM5 and making a V56A mutation in repA.


Source

The pSC101ts origin was cloned from the pSIM5 plasmid from Datta et al, see reference below.

References

[http://dx.doi.org/10.1016/j.gene.2006.04.018] Datta, S., Costantino, N. & Court, D.L. A set of recombineering plasmids for gram-negative bacteria. Gene 379, 109–115 (2006).