Difference between revisions of "Part:BBa K864445:Experience"
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The primers that were used in order to perform the site directed mutagenesis to replace the antisense region with a random nucleotide sequence showed to contain a problematic secondary structure. Be sure to check https://parts.igem.org/Part:BBa_K864440:Experience to see the PCR program and PCR protocol that was used to bypass the problems with the secondary structure. | The primers that were used in order to perform the site directed mutagenesis to replace the antisense region with a random nucleotide sequence showed to contain a problematic secondary structure. Be sure to check https://parts.igem.org/Part:BBa_K864440:Experience to see the PCR program and PCR protocol that was used to bypass the problems with the secondary structure. | ||
Latest revision as of 21:58, 29 September 2012
The primers that were used in order to perform the site directed mutagenesis to replace the antisense region with a random nucleotide sequence showed to contain a problematic secondary structure. Be sure to check https://parts.igem.org/Part:BBa_K864440:Experience to see the PCR program and PCR protocol that was used to bypass the problems with the secondary structure.
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