Difference between revisions of "Part:BBa K779406"
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| − | + | The U6-TetO-TuDFF4-3p part encodes conditional expression of the short RNA sequence TuDFF4-3p. The mammalian RNA Pol III U6 promoter incorporates tetracycline-responsive elements that allow for tightly regulated RNA expression under specific conditions. In the presence of the Tet repressor (TetR), two Tet operator (TetO) sites become bound by the TetR and prevent RNA Pol III from binding, subsequently inhibiting transcription of the downstream sequence. In the absence of TetR, RNA transcription proceeds normally. The short TuDFF4-3p RNA sequence can be used as an RNA interference mechanism through its binding to a complementary region present in the micro RNA sequence FF4 (miR-FF4), thus inhibiting the RNA interference activity of the miR-FF4. Ultimately, the gene that miR-FF4 generally silences is allowed to be expressed. | |
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Latest revision as of 20:47, 29 September 2012
U6-TetO-TuDFF4-3p MammoBlock
The U6-TetO-TuDFF4-3p part encodes conditional expression of the short RNA sequence TuDFF4-3p. The mammalian RNA Pol III U6 promoter incorporates tetracycline-responsive elements that allow for tightly regulated RNA expression under specific conditions. In the presence of the Tet repressor (TetR), two Tet operator (TetO) sites become bound by the TetR and prevent RNA Pol III from binding, subsequently inhibiting transcription of the downstream sequence. In the absence of TetR, RNA transcription proceeds normally. The short TuDFF4-3p RNA sequence can be used as an RNA interference mechanism through its binding to a complementary region present in the micro RNA sequence FF4 (miR-FF4), thus inhibiting the RNA interference activity of the miR-FF4. Ultimately, the gene that miR-FF4 generally silences is allowed to be expressed.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]