Difference between revisions of "Part:BBa K776019:Experience"
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===Applications of BBa_K776019=== | ===Applications of BBa_K776019=== | ||
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| − | The PrrA dependent promoter was used in purple non-sulfur photosynthetic bacteria ''R. sphaeroides'' and '' R. palustris'', using different conditions. For test the promoter we used as a reporter GFP. | + | |
| + | == '''iGEM CINVESTAV_IPN-UNAM''' == | ||
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| + | The PrrA dependent promoter was used in purple non-sulfur photosynthetic bacteria (PNSP) ''R. sphaeroides'' and ''R. palustris'', using different conditions. For test the promoter we used as a reporter GFP. | ||
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| + | [[Image:promprra.jpg]] | ||
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| + | ''Fig 1. Construction of PrrA dependent promoter.'' | ||
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| + | We employed flow cytometery for mesuring the expression of GFP. The conditions we used for test the PrrA dependent promoter were: aerobic/darness, anaerobic/light, anaerobic/darknness. The results were as follows. | ||
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| + | '''''R. sphaeroides''''' | ||
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| + | [[Image:as1.jpg]] | ||
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| + | ''Graphic 1.: Quantitative analysis of bacterial population with GFP expression (GFP+), under above condition, using flow cytometry (Attune cytometer Applied Biosystems), background signals were eliminated using a negative control.'' | ||
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| + | [[Image:as2.jpg]] | ||
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| + | ''Graphic 2. Median fluorescence Intensity of the conjugated R. sphaeroides strain. Analysis of fluorescence in bacterial populations (1000 bacteria), under above conditions, using flow cytometry (Attune cytometer Applied Biosystems), background signals were eliminated using a negative control.'' | ||
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| + | '''''R. palustris''''' | ||
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| + | [[Image:ap1.jpg]] | ||
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| + | ''Graphic 3.: Quantitative analysis of bacterial population with GFP expression (GFP+), under above condition, using flow cytometry (Attune cytometer Applied Biosystems), background signals were eliminated using a negative control.'' | ||
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| + | [[Image:ap2.jpg]] | ||
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| + | ''Graphic 4. Median fluorescence Intensity of the conjugated R. palustris strain. Analysis of fluorescence in bacterial populations (1000 bacteria), under above conditions, using flow cytometry (Attune cytometer Applied Biosystems), background signals were eliminated using a negative control.'' | ||
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| + | Results above shows that the PrrA dependent promoter is functional in our two PNSP bacteria. | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 19:53, 29 September 2012
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K776019
iGEM CINVESTAV_IPN-UNAM
The PrrA dependent promoter was used in purple non-sulfur photosynthetic bacteria (PNSP) R. sphaeroides and R. palustris, using different conditions. For test the promoter we used as a reporter GFP.
Fig 1. Construction of PrrA dependent promoter.
We employed flow cytometery for mesuring the expression of GFP. The conditions we used for test the PrrA dependent promoter were: aerobic/darness, anaerobic/light, anaerobic/darknness. The results were as follows.
R. sphaeroides
Graphic 1.: Quantitative analysis of bacterial population with GFP expression (GFP+), under above condition, using flow cytometry (Attune cytometer Applied Biosystems), background signals were eliminated using a negative control.
Graphic 2. Median fluorescence Intensity of the conjugated R. sphaeroides strain. Analysis of fluorescence in bacterial populations (1000 bacteria), under above conditions, using flow cytometry (Attune cytometer Applied Biosystems), background signals were eliminated using a negative control.
R. palustris
Graphic 3.: Quantitative analysis of bacterial population with GFP expression (GFP+), under above condition, using flow cytometry (Attune cytometer Applied Biosystems), background signals were eliminated using a negative control.
Graphic 4. Median fluorescence Intensity of the conjugated R. palustris strain. Analysis of fluorescence in bacterial populations (1000 bacteria), under above conditions, using flow cytometry (Attune cytometer Applied Biosystems), background signals were eliminated using a negative control.
Results above shows that the PrrA dependent promoter is functional in our two PNSP bacteria.
User Reviews
UNIQ873e0b7c947aea6e-partinfo-00000000-QINU UNIQ873e0b7c947aea6e-partinfo-00000001-QINU