Difference between revisions of "Part:BBa K864120"
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__NOTOC__ | __NOTOC__ | ||
| − | [[Image:BBa_K864120onplate.JPG | + | [[Image:BBa_K864120onplate.JPG|thumb|Appearance of pUCori-red, in pSB4K15 and DH5α, on LA plate.]] |
<partinfo>BBa_K864120 short</partinfo> | <partinfo>BBa_K864120 short</partinfo> | ||
| − | This is a minimal high-copy replication origin coupled to the standard RFP | + | This is a minimal high-copy replication origin coupled to the standard RFP cassette <partinfo>J04450</partinfo>. Any vector containing K864120 will be propagated at high copy and express a strong red color after 12-18 hours of incubation. Also known as "pUCori-red". |
* LacI sensitive | * LacI sensitive | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
| − | <partinfo> | + | <partinfo>BBa_K864120 SequenceAndFeatures</partinfo> |
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Latest revision as of 18:42, 29 September 2012
pMB1 ori - RFP cassette
This is a minimal high-copy replication origin coupled to the standard RFP cassette BBa_J04450. Any vector containing K864120 will be propagated at high copy and express a strong red color after 12-18 hours of incubation. Also known as "pUCori-red".
- LacI sensitive
- CAP sensitive
Usage and Biology
Usable as default insert of low and medium copy backbones. This is the default insert of the pSB4x15 series of backbones.
The constitutive version of this part, BBa_K864121, may be more practical for most applications.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 19
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1463
Illegal AgeI site found at 1575 - 1000COMPATIBLE WITH RFC[1000]