Difference between revisions of "Part:BBa K864120"
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K864121 short</partinfo>
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[[Image:BBa_K864120onplate.JPG|thumb|Appearance of pUCori-red, in pSB4K15 and DH5α, on LA plate.]]
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<partinfo>BBa_K864120 short</partinfo>
  
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This is a minimal high-copy replication origin coupled to the standard RFP casette <partinfo>J04450</partinfo>. Any vector containing K864120 will be propagated at high copy and express a strong red color after 12-18 hours of incubation. Also known as "pMB1ori-red".  
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This is a minimal high-copy replication origin coupled to the standard RFP cassette <partinfo>J04450</partinfo>. Any vector containing K864120 will be propagated at high copy and express a strong red color after 12-18 hours of incubation. Also known as "pUCori-red".  
  
 
* LacI sensitive
 
* LacI sensitive
 
* CAP sensitive
 
* CAP sensitive
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===Usage and Biology===
 
===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K864121 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K864120 SequenceAndFeatures</partinfo>
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K864121 parameters</partinfo>
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<partinfo>BBa_K864120 parameters</partinfo>
 
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Latest revision as of 18:42, 29 September 2012

Appearance of pUCori-red, in pSB4K15 and DH5α, on LA plate.

pMB1 ori - RFP cassette

This is a minimal high-copy replication origin coupled to the standard RFP cassette BBa_J04450. Any vector containing K864120 will be propagated at high copy and express a strong red color after 12-18 hours of incubation. Also known as "pUCori-red".

  • LacI sensitive
  • CAP sensitive


Usage and Biology

Usable as default insert of low and medium copy backbones. This is the default insert of the pSB4x15 series of backbones.

The constitutive version of this part, BBa_K864121, may be more practical for most applications.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 19
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1463
    Illegal AgeI site found at 1575
  • 1000
    COMPATIBLE WITH RFC[1000]