Difference between revisions of "Part:BBa K864100:Experience"

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1. As a part of the sRNA screening system target construct (<partinfo>K864444</partinfo>), where it was a reporter of translational downregulation. Thanks to the very strong fluorescence, cells with very low levels of expression could easily be distinguished from non-fluorescent cells, using a FACS. In this experiment it was fused to a part of another protein, with the <partinfo>J18922</partinfo> gly-ser linker in between. Fluorescence was not affected by this fusion.  
 
1. As a part of the sRNA screening system target construct (<partinfo>K864444</partinfo>), where it was a reporter of translational downregulation. Thanks to the very strong fluorescence, cells with very low levels of expression could easily be distinguished from non-fluorescent cells, using a FACS. In this experiment it was fused to a part of another protein, with the <partinfo>J18922</partinfo> gly-ser linker in between. Fluorescence was not affected by this fusion.  
  
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2. In our promoter test, where it was coupled to different promoter as promoter-B0032-SYFP2 (using <partinfo>K864101</partinfo>).  
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2. In our promoter test, where it was coupled to different promoter as promoters-B0032-SYFP2 (using <partinfo>K864101</partinfo>).  
 
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Revision as of 18:30, 29 September 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K864100

User Reviews

UNIQ17c6310cb6a98d72-partinfo-00000000-QINU

No review score entered. User:agynna

iGEM Team Uppsala University 2012

This part was used as an fluorescent reporter in two ways by the Uppsala iGEM team 2012. It could easily be observed both in a Fluorescence activated cell sorter (FACS), on a UV table and even better on a Visi-Blue blue-light table. We recommend it whenever a strong fluorescent reporter is needed.

1. As a part of the sRNA screening system target construct (BBa_K864444), where it was a reporter of translational downregulation. Thanks to the very strong fluorescence, cells with very low levels of expression could easily be distinguished from non-fluorescent cells, using a FACS. In this experiment it was fused to a part of another protein, with the BBa_J18922 gly-ser linker in between. Fluorescence was not affected by this fusion.

2. In our promoter test, where it was coupled to different promoter as promoters-B0032-SYFP2 (using BBa_K864101).

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UNIQ17c6310cb6a98d72-partinfo-00000005-QINU