Difference between revisions of "Part:BBa K845000"
 
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===Usage and Biology===
 
===Usage and Biology===
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paraBAD is a promoter which can be activated by 2 molecules : CRP-cAMP complex and the AraC protein. It has two states; in absence of L-arabinose the paraBAD promoter is repressed by AraC, whereas the paraC promoter is activated (unless an excess of AraC is present).
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In presence of L-arabinose and the CRP-cAMP complex, the promoter is activated thus enabling the transcription of the downstream elements.
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This AND gate provides a filter to biological noise.
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K845000 parameters</partinfo>
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<partinfo>BBa_K845001 parameters</partinfo>
 
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Latest revision as of 17:00, 29 September 2012

iGEM 2012 Grenoble Team proposes a new design and application to the pBAD promoter(paraBAD).

In order to create a biological AND gate the 2012 Grenoble team worked on the cAMP-CRP complex and arabinose inductible promoter.

The activity of the promoter was tested under several conditions ( http://2012.igem.org/Team:Grenoble/Biology/Protocols/AND_test ) using a GFP as a reporter (on a pUA66). We tested this activity in BW25113 ΔcyaA in different culture media (Glucose + Acetate; Glycerol; Acetate).



Usage and Biology

paraBAD is a promoter which can be activated by 2 molecules : CRP-cAMP complex and the AraC protein. It has two states; in absence of L-arabinose the paraBAD promoter is repressed by AraC, whereas the paraC promoter is activated (unless an excess of AraC is present). In presence of L-arabinose and the CRP-cAMP complex, the promoter is activated thus enabling the transcription of the downstream elements. This AND gate provides a filter to biological noise.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 500
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 344
    Illegal XhoI site found at 494
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 500
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 500
    Illegal AgeI site found at 179
    Illegal AgeI site found at 484
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1169
    Illegal SapI site found at 161