Difference between revisions of "Part:BBa K861024:Experience"
(→‎Applications of BBa_K861024)
 
(One intermediate revision by one other user not shown)
Line 10: Line 10:
  
 
<I>iGEM2012 WHU-China</I>
 
<I>iGEM2012 WHU-China</I>
−
<p>In the in vitro assay we performed, we collected cell extracts from 1L medium and got protein solution of high concentration .The enzyme assay was then performed to measure the ability of degrading fatty acids by single enzyme and their different combinations.The remaining amount of fatty acids in the medium is measured by the cupric-acetate reaction .
+
<p>In the in vitro assay we performed, we collected cell extracts from 1L medium and got protein solution of high concentration. The enzyme assay was then performed to measure the ability of degrading fatty acids by single enzyme and their different combinations.The remaining amount of fatty acids in the medium was measured by the cupric-acetate reaction.
  
 
https://static.igem.org/mediawiki/parts/b/bc/Cupric_acetate-1-.png
 
https://static.igem.org/mediawiki/parts/b/bc/Cupric_acetate-1-.png
 +
https://static.igem.org/mediawiki/parts/d/d9/%E4%BD%93%E5%A4%96%E5%AE%9E%E9%AA%8C.jpg<br/>
  
−
In this picture , the color of the sample   D   standing for cell extracts collected from bacteria overexpressing fadE is lighter than the control(tube A) . It means the fatty acids remaining in the tube is less .From the results above, we have proved that the biobrick responsible for overexpressing the mentioned gene can metabolize fatty acids much faster.
+
 
 +
In this picture, the color of the sample D standing for cell extracts collected from bacteria overexpressing fadE was lighter than the control(tube A). It means the fatty acids remaining in the tube was less. From the results above, we have proved that the biobrick responsible for overexpressing the mentioned gene can metabolize fatty acids much faster.
  
 
For more details, please visit our wiki http://2012.igem.org/Team:WHU-China
 
For more details, please visit our wiki http://2012.igem.org/Team:WHU-China

Latest revision as of 16:56, 29 September 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K861024

•

iGEM2012 WHU-China

In the in vitro assay we performed, we collected cell extracts from 1L medium and got protein solution of high concentration. The enzyme assay was then performed to measure the ability of degrading fatty acids by single enzyme and their different combinations.The remaining amount of fatty acids in the medium was measured by the cupric-acetate reaction. Cupric_acetate-1-.png %E4%BD%93%E5%A4%96%E5%AE%9E%E9%AA%8C.jpg
In this picture, the color of the sample D standing for cell extracts collected from bacteria overexpressing fadE was lighter than the control(tube A). It means the fatty acids remaining in the tube was less. From the results above, we have proved that the biobrick responsible for overexpressing the mentioned gene can metabolize fatty acids much faster. For more details, please visit our wiki http://2012.igem.org/Team:WHU-China

;

User Reviews

UNIQdd88f918ebe41848-partinfo-00000001-QINU

UNIQdd88f918ebe41848-partinfo-00000002-QINU