Difference between revisions of "Part:BBa K845000:Experience"
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In order to characterize the paraBAD promoter we used a transcriptional fusion of the promoter and gfpmut2 from Alon on pUA66 in BW25113 ΔcyaA. | In order to characterize the paraBAD promoter we used a transcriptional fusion of the promoter and gfpmut2 from Alon on pUA66 in BW25113 ΔcyaA. | ||
We followed the fluorescent expression of the GFP versus different concentrations of arabinose AND cAMP over time. We made this experiment in different growth media: | We followed the fluorescent expression of the GFP versus different concentrations of arabinose AND cAMP over time. We made this experiment in different growth media: | ||
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M9 complement with 0.03% of glucose and 0.03% of acetate (1) | M9 complement with 0.03% of glucose and 0.03% of acetate (1) | ||
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M9 complement with 0.1% of glycerol (2) | M9 complement with 0.1% of glycerol (2) | ||
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M9 complement with 0.1% of acetate (3) | M9 complement with 0.1% of acetate (3) | ||
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See the protocol: http://2012.igem.org/Team:Grenoble/Biology/Protocols/AND_test | See the protocol: http://2012.igem.org/Team:Grenoble/Biology/Protocols/AND_test | ||
Revision as of 15:21, 29 September 2012
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Applications of BBa_K845000
In order to characterize the paraBAD promoter we used a transcriptional fusion of the promoter and gfpmut2 from Alon on pUA66 in BW25113 ΔcyaA. We followed the fluorescent expression of the GFP versus different concentrations of arabinose AND cAMP over time. We made this experiment in different growth media:
M9 complement with 0.03% of glucose and 0.03% of acetate (1)
M9 complement with 0.1% of glycerol (2)
M9 complement with 0.1% of acetate (3)
See the protocol: http://2012.igem.org/Team:Grenoble/Biology/Protocols/AND_test
Those different media did not affect bacterial growth.
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