Difference between revisions of "Part:BBa K772100"

Revision as of 13:57, 29 September 2012

EpCAM Detection Device

This gene sequence includes both of EpCAM Binding Docks; BBa_K772001[1] and BBa_K772002[2]. Thus, this composite part is the main component of CTC (Circulating Tumor Cell) detection system. Docks include an EpCAM (Epithelial Cell Adhesion Molecule) antigen binding domain, OmpA transmembrane protein (dock) and TEV protease C or N terminus. Hopefully, after binding to the EpCAM antigen, TEV protease domains will fix together to form the full structure in order to activate the signalling pathway. (For more information: BBa_K772003[3]) EpCAM is an natural antigen which is found high significantly in the cases of some breast and colorectal cancer subtypes. On the upstream region of protein sequences, there are a constitutive pLac promoter and appropriate RBS.

To see how this part works: [4]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 13
Illegal NheI site found at 36
Illegal NheI site found at 1180
Illegal NheI site found at 1203
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 441
Illegal SapI.rc site found at 1614

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K772100 short</partinfo>
-This gene sequence includes both of EpCAM Binding Docks; BBa_K772001 and BBa_K772002.
+This gene sequence includes both of EpCAM Binding Docks; BBa_K772001[https://parts.igem.org/wiki/index.php?title=Part:BBa_K772001] and BBa_K772002[https://parts.igem.org/wiki/index.php?title=Part:BBa_K772002].
-Thus, this composite part is the main component of CTC (Circulating Tumor Cell) detection system. Docks include an EpCAM (Epithelial Cell Adhesion Molecule) antigen binding domain, OmpA transmembrane protein (dock) and TEV protease C or N terminus. Hopefully, after binding to the EpCAM antigen, TEV protease domains will fix together to form the full structure in order to activate the signalling pathway. (For more information: BBa_K772003)
+Thus, this composite part is the main component of CTC (Circulating Tumor Cell) detection system. Docks include an EpCAM (Epithelial Cell Adhesion Molecule) antigen binding domain, OmpA transmembrane protein (dock) and TEV protease C or N terminus. Hopefully, after binding to the EpCAM antigen, TEV protease domains will fix together to form the full structure in order to activate the signalling pathway. (For more information: BBa_K772003[https://parts.igem.org/wiki/index.php?title=Part:BBa_K772003])
 EpCAM is an natural antigen which is found high significantly in the cases of some breast and colorectal cancer subtypes.
 On the upstream region of protein sequences, there are a constitutive pLac promoter and appropriate RBS.