Difference between revisions of "Part:BBa K771107"
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===Usage and Biology=== | ===Usage and Biology=== | ||
− | [[Image: | + | [[Image:12SJTU SH3dl.gif|right]] |
− | The | + | The SH3 domain along with its ligand([https://parts.igem.org/Part:BBa_K771108 Part:BBa_K771108]) comes from ''Mus musculus'', which could dimerize spontaneously. The original dimer was introduced by Dueber et al., 2012. This pair of dimer is a perfect material to construct the dimerizable proteins. In Membrane Magic system designed by [http://2012.igem.org/Team:SJTU-BioX-Shanghai 2012 SJTU-BioX-Shanghai Team], the SH3 Domain is located in Membrane Anchor 2, while the SH3 ligand on Membrane Anchor 3. A flexible linker3(FL3) is added to the N-terminus of the domain, enabling its proper interaction with its domain on Membrane Anchor 3. |
===Related Parts=== | ===Related Parts=== | ||
− | |||
− | [https://parts.igem.org/Part: | + | [https://parts.igem.org/Part:BBa_K771106 Part:BBa_K771108, SH3 ligand] |
+ | |||
+ | |||
+ | [https://parts.igem.org/Part:BBa_K771004 Part:BBa_K771002, Membrane Anchor 2, containing SH3 domain] | ||
+ | |||
+ | |||
+ | [https://parts.igem.org/Part:BBa_K771005 Part:BBa_K771003, Membrane Anchor 3, containing SH3 ligand] | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K771107 SequenceAndFeatures</partinfo> | <partinfo>BBa_K771107 SequenceAndFeatures</partinfo> |
Latest revision as of 04:03, 29 September 2012
SH3domain
Components of interacting proteins
Usage and Biology
The SH3 domain along with its ligand(Part:BBa_K771108) comes from Mus musculus, which could dimerize spontaneously. The original dimer was introduced by Dueber et al., 2012. This pair of dimer is a perfect material to construct the dimerizable proteins. In Membrane Magic system designed by [http://2012.igem.org/Team:SJTU-BioX-Shanghai 2012 SJTU-BioX-Shanghai Team], the SH3 Domain is located in Membrane Anchor 2, while the SH3 ligand on Membrane Anchor 3. A flexible linker3(FL3) is added to the N-terminus of the domain, enabling its proper interaction with its domain on Membrane Anchor 3.
Related Parts
Part:BBa_K771002, Membrane Anchor 2, containing SH3 domain
Part:BBa_K771003, Membrane Anchor 3, containing SH3 ligand
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 145