Difference between revisions of "Part:BBa K771105"
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<partinfo>BBa_K771105 short</partinfo> | <partinfo>BBa_K771105 short</partinfo> | ||
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Components of interacting proteins | Components of interacting proteins | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | [[Image:12SJTU GBDdl.gif|right]] | ||
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+ | The GBD domain along with its ligand([https://parts.igem.org/Part:BBa_K771106 Part:BBa_K771106]) comes from ''Rattus rattus'', which could dimerize spontaneously. The original dimer was introduced by Dueber et al., 2012. This pair of dimer is a perfect material to construct the dimerizable proteins. In Membrane Magic system designed by [http://2012.igem.org/Team:SJTU-BioX-Shanghai 2012 SJTU-BioX-Shanghai Team], the GBD domain is located in Membrane Anchor 4, while the GBD ligand on Membrane Anchor 5. A flexible linker3(FL3) is added to the N-terminus of the domain, enabling its proper interaction with its domain on Membrane Anchor 4. | ||
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+ | ===Related Parts=== | ||
+ | [https://parts.igem.org/Part:BBa_K771106 Part:BBa_K771106, GBD ligand] | ||
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+ | [https://parts.igem.org/Part:BBa_K771004 Part:BBa_K771004, Membrane Anchor 4, containing GBD domain] | ||
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+ | [https://parts.igem.org/Part:BBa_K771005 Part:BBa_K771005, Membrane Anchor 5, containing GBD ligand] | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K771105 SequenceAndFeatures</partinfo> | <partinfo>BBa_K771105 SequenceAndFeatures</partinfo> |
Latest revision as of 03:57, 29 September 2012
GBDdomain
Components of interacting proteins
Usage and Biology
The GBD domain along with its ligand(Part:BBa_K771106) comes from Rattus rattus, which could dimerize spontaneously. The original dimer was introduced by Dueber et al., 2012. This pair of dimer is a perfect material to construct the dimerizable proteins. In Membrane Magic system designed by [http://2012.igem.org/Team:SJTU-BioX-Shanghai 2012 SJTU-BioX-Shanghai Team], the GBD domain is located in Membrane Anchor 4, while the GBD ligand on Membrane Anchor 5. A flexible linker3(FL3) is added to the N-terminus of the domain, enabling its proper interaction with its domain on Membrane Anchor 4.
Related Parts
Part:BBa_K771004, Membrane Anchor 4, containing GBD domain
Part:BBa_K771005, Membrane Anchor 5, containing GBD ligand
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]