Difference between revisions of "Part:BBa K771106"

 
 
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<partinfo>BBa_K771106 short</partinfo>
 
<partinfo>BBa_K771106 short</partinfo>
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components of interacting proteins
 
components of interacting proteins
  
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===Usage and Biology===
 
===Usage and Biology===
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[[Image:12SJTU GBDdl.gif|right]]
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The GBD ligand along with its domain([https://parts.igem.org/Part:BBa_K771105 Part:BBa_K771105]) comes from ''Rattus rattus'', which could dimerize spontaneously. The original dimer was introduced by Dueber et al., 2012. This pair of dimer is a perfect material to construct the dimerizable proteins. In Membrane Magic system designed by [http://2012.igem.org/Team:SJTU-BioX-Shanghai 2012 SJTU-BioX-Shanghai Team], the GBD ligand is located in Membrane Anchor 5, while the GBD domain on Membrane Anchor 4. A flexible linker3(FL3) is added to the N-terminus of the domain, enabling its proper interaction with its domain on Membrane Anchor 4.
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===Related Parts===
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[https://parts.igem.org/Part:BBa_K771106 Part:BBa_K771105, GBD domain]
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[https://parts.igem.org/Part:BBa_K771004 Part:BBa_K771004, Membrane Anchor 4, containing GBD domain]
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[https://parts.igem.org/Part:BBa_K771005 Part:BBa_K771005, Membrane Anchor 5, containing GBD ligand]
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K771106 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K771106 SequenceAndFeatures</partinfo>

Latest revision as of 03:56, 29 September 2012

GBDligand

components of interacting proteins

Usage and Biology

12SJTU GBDdl.gif

The GBD ligand along with its domain(Part:BBa_K771105) comes from Rattus rattus, which could dimerize spontaneously. The original dimer was introduced by Dueber et al., 2012. This pair of dimer is a perfect material to construct the dimerizable proteins. In Membrane Magic system designed by [http://2012.igem.org/Team:SJTU-BioX-Shanghai 2012 SJTU-BioX-Shanghai Team], the GBD ligand is located in Membrane Anchor 5, while the GBD domain on Membrane Anchor 4. A flexible linker3(FL3) is added to the N-terminus of the domain, enabling its proper interaction with its domain on Membrane Anchor 4.

Related Parts

Part:BBa_K771105, GBD domain


Part:BBa_K771004, Membrane Anchor 4, containing GBD domain


Part:BBa_K771005, Membrane Anchor 5, containing GBD ligand


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]