Difference between revisions of "Part:BBa K771104"

Latest revision as of 03:31, 29 September 2012

ssDsbA-PDZligand

components of interacting proteins

Usage and Biology

The PDZ ligand along with its domain(Part:BBa_K771103) comes from Mus musculus, which could dimerize spontaneously. The original dimer was introduced by Dueber et al., 2012. This pair of dimer is a perfect material to construct the dimerizable proteins. In Membrane Magic system designed by [http://2012.igem.org/Team:SJTU-BioX-Shanghai 2012 SJTU-BioX-Shanghai Team], the PDZ ligand is located in Membrane Anchor 3, while the PDZ domain on Membrane Anchor 4. Due to the fact that the PDZ domain and ligand is in the N-terminus of the whole protein, we added a SRP-dependent signal peptide, ssdsbA, to help the Membrane Anchor localize to the membrane correctly.

Related Parts

Part:BBa_K771103, PDZ domain

Part:BBa_K771003, Membrane Anchor 3, containing PDZ ligand

Part:BBa_K771004, Membrane Anchor 4, containing PDZ domaim

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 [[Image:12SJTU_2PDZ.gif|right]]
-The PDZ domain along with its ligand([https://parts.igem.org/Part:BBa_K771104 Part:BBa_K771104]) comes from ''Mus musculus'', which could dimerize spontaneously. The original dimer was introduced by Dueber et al., 2012. This pair of dimer is a perfect material to construct the dimerizable proteins. In Membrane Magic system designed by [http://2012.igem.org/Team:SJTU-BioX-Shanghai 2012 SJTU-BioX-Shanghai Team], the PDZ domain is located in Membrane Anchor 4, while the PDZ ligands on Membrane Anchor 3. Due to the fact that the PDZ domain and ligand is in the N-terminus of the whole protein, we added a SRP-dependent signal peptide, ssdsb, to help the Membrane Anchor localize to the membrane correctly.
+The PDZ ligand along with its domain([https://parts.igem.org/Part:BBa_K771103 Part:BBa_K771103]) comes from ''Mus musculus'', which could dimerize spontaneously. The original dimer was introduced by Dueber et al., 2012. This pair of dimer is a perfect material to construct the dimerizable proteins. In Membrane Magic system designed by [http://2012.igem.org/Team:SJTU-BioX-Shanghai 2012 SJTU-BioX-Shanghai Team], the PDZ ligand is located in Membrane Anchor 3, while the PDZ domain on Membrane Anchor 4. Due to the fact that the PDZ domain and ligand is in the N-terminus of the whole protein, we added a SRP-dependent signal peptide, ssdsbA, to help the Membrane Anchor localize to the membrane correctly.
 ===Related Parts===
-[https://parts.igem.org/Part:BBa_K771104 Part:BBa_K771104, PDZ ligand]
+[https://parts.igem.org/Part:BBa_K771103 Part:BBa_K771103, PDZ domain]
 [https://parts.igem.org/Part:BBa_K771003 Part:BBa_K771003, Membrane Anchor 3, containing PDZ ligand]