Difference between revisions of "Part:BBa K900000:Design"
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<partinfo>BBa_K900000 short</partinfo>
 
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PAmCHERRY is a photoactivatable red fluorescent protein. PAmCherry fluoresces when exposed to 350–400 nm light while does not fluoresce at other wavelengths. It is used a reporter tag; by expressing in cells or localizing to sub-cellular locations, one can gain spatial information within the cell by track organelles, proteins, and the whole cell itself.  One can visualize the photoactivated PAmCherry with the same filter sets used for other red fluorescent proteins.
 
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<partinfo>BBa_K900000 SequenceAndFeatures</partinfo>
 
  
  
 
===Design Notes===
 
===Design Notes===
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Codon optimization for yeast and internal restriction site removal to be compatible with our Golden Gate cloning scheme.  
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The gene was codon optimized for yeast and internal restriction sites were removed for to compatibility with our Golden Gate cloning scheme.  
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Revision as of 21:39, 28 September 2012


Design Notes

The gene was codon optimized for yeast and internal restriction sites were removed for to compatibility with our Golden Gate cloning scheme.


Source

Synthesized in house. Non-genomic DNA sequence. Mutated version of RFP.

References