Difference between revisions of "Part:BBa K942013:Design"
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===Source=== | ===Source=== | ||
− | The Raghium inquisitor Anti Freeze Protein (RiAFP) is a 137 AA protein that prevents the growth of ice crystals. This protein is produced natively in the beetle Raghium inquisitor. This part includes the OmpA signal sequence native from E.coli and the pBAD L-arabinose inducible promoter. (Kristiansen E, 2011) | + | The Raghium inquisitor Anti Freeze Protein (RiAFP) is a 137 AA protein that prevents the growth of ice crystals. This protein is produced natively in the beetle Raghium inquisitor. This part includes the OmpA signal sequence native from E.coli and the pBAD L-arabinose inducible promoter. (Kristiansen E, 2011) |
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===References=== | ===References=== |
Revision as of 21:35, 28 September 2012
pBAD+OmpA+RiAFP+Term
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
Illegal AgeI site found at 1363
Illegal AgeI site found at 1489
Illegal AgeI site found at 1573 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Design Notes
The RiAFP is better expressed when induced with 0.2-0.4% L-Arabinose. This part was designed by adding a 6xHis tag for affinity purification to the anti freeze protein from Raghium inquisitor and the signal sequence of the ompA gene for periplasmic secretion. This part was codon optimized for its expression in E. coli.
Source
The Raghium inquisitor Anti Freeze Protein (RiAFP) is a 137 AA protein that prevents the growth of ice crystals. This protein is produced natively in the beetle Raghium inquisitor. This part includes the OmpA signal sequence native from E.coli and the pBAD L-arabinose inducible promoter. (Kristiansen E, 2011)