Difference between revisions of "Part:BBa K540000:Experience"
(→‎User Reviews)
 
(45 intermediate revisions by 3 users not shown)
Line 4: Line 4:
  
 
===Applications of BBa_K540000===
 
===Applications of BBa_K540000===
−
<html>
 
−
<div style="text-align:center; float:right; margin-left:30px; margin-top:-20px;">
 
−
<img src="http://i.imgur.com/g7iTW.png" style="width:340px; height:255px; margin-top: 70px; margin-bottom: 5px; position: relative;"/>
 
−
</div>
 
−
<p>
 
−
A preliminary search on the best condition for use was carried out at first.
 
−
</p><br>
 
−
 
−
<p>
 
−
<FONT COLOR="green"><b>Antibiotic response</b></FONT><br>
 
−
Two 24 wells plates have been seeded with the same strains, in LB/2 medium. Ampicilline for S18 and Cm have been added only in the second plate.
 
−
The results have examined after 24 hours of incubation 30°C.
 
−
</p><br>
 
−
 
−
<p><i>Qualitative approach</i><br>
 
−
As can be seen on the right, adding ampicilline boosts the adherence of the strain, and makes it more cobalt responsive.
 
−
</p>
 
−
 
−
 
−
<div style="text-align:center; float:left; margin-left:30px; margin-top:-20px;">
 
−
<img src="http://i.imgur.com/wnOY5.png" style="width:525px; height:350px; margin-top: 90px; margin-bottom: 5px; position: relative;"/>
 
−
</div>
 
−
 
−
<br><br><p>
 
−
<i>Quantitative approach</i><br>
 
−
Furthermore, the adherence level was determined quantitatively using OD measurements. Resulting graphs are shown on the left.
 
−
</p>
 
−
 
−
 
−
<br/><br/><br/><br/>
 
  
 
===User Reviews===
 
===User Reviews===
Line 39: Line 9:
 
<!-- Template for a user review
 
<!-- Template for a user review
 
{|width='80%' style='border:1px solid gray'
 
{|width='80%' style='border:1px solid gray'
−
|-
+
|-Team
 
|width='10%'|
 
|width='10%'|
 
<partinfo>BBa_K540000 AddReview number</partinfo>
 
<partinfo>BBa_K540000 AddReview number</partinfo>
−
<I>Username</I>
+
<I>User</I>
 
|width='60%' valign='top'|
 
|width='60%' valign='top'|
−
Enter the review inofrmation here.
+
 
 
|};
 
|};
 
<!-- End of the user review template -->
 
<!-- End of the user review template -->
 
<!-- DON'T DELETE --><partinfo>BBa_K540000 EndReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K540000 EndReviews</partinfo>
 +
 +
{|width='80%' style='border:1px solid gray'
 +
|-
 +
|width='10%'|
 +
<partinfo>BBa_I0500 AddReview 5</partinfo>
 +
<I>UCL iGEM 2012</I>
 +
|width='60%' valign='top'|
 +
 +
UCL iGEM 2012 have removed the original cobalt inducible promoter to create a stand alone csgBAEFG operon with rbs [https://parts.igem.org/wiki/index.php?title=Part:BBa_K729003 BBa_K729003]. We have also designed two additional variations on the part, with a [https://parts.igem.org/wiki/index.php?title=Part:BBa_K729017 T7] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K729018 constitutive promoter].
 +
 +
|}
 +
{|width='80%' style='border:1px solid gray'
 +
|-

Latest revision as of 19:30, 28 September 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K540000

User Reviews

UNIQ53ac5828a61cb47a-partinfo-00000000-QINU UNIQ53ac5828a61cb47a-partinfo-00000001-QINU

•••••

UCL iGEM 2012

UCL iGEM 2012 have removed the original cobalt inducible promoter to create a stand alone csgBAEFG operon with rbs BBa_K729003. We have also designed two additional variations on the part, with a T7 and constitutive promoter.