Difference between revisions of "Part:BBa K540000:Experience"
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===Applications of BBa_K540000===
 
===Applications of BBa_K540000===
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<div style="text-align:center; float:right; margin-left:30px; margin-top:-20px;">
 
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<img src="http://i.imgur.com/g7iTW.png" style="width:340px; height:255px; margin-top: 70px; margin-bottom: 5px; position: relative;"/>
 
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<p>
 
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A preliminary search on the best condition for use was carried out at first.
 
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</p><br>
 
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<p>
 
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<FONT COLOR="green"><b>Antibiotic response</b></FONT><br>
 
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Two 24 wells plates have been seeded with the same strains, in LB/2 medium. Ampicilline for S18 and Cm have been added only in the second plate.
 
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The results have examined after 24 hours of incubation 30°C.
 
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</p><br>
 
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<p><i>Qualitative approach</i><br>
 
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As can be seen on the right, adding ampicilline boosts the adherence of the strain, and makes it more cobalt responsive.
 
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<div style="text-align:center; float:left; margin-left:30px; margin-top:-20px;">
 
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<img src="http://i.imgur.com/wnOY5.png" style="width:525px; height:350px; margin-top: 90px; margin-bottom: 5px; position: relative;"/>
 
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</div>
 
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<br><br><p>
 
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<i>Quantitative approach</i><br>
 
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Furthermore, the adherence level was determined quantitatively using OD measurements. Resulting graphs are shown on the left.
 
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<br/><br/><br/><br/>
 
  
 
===User Reviews===
 
===User Reviews===
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<partinfo>BBa_K540000 AddReview number</partinfo>
 
<partinfo>BBa_K540000 AddReview number</partinfo>
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<I>Username</I>
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<I>User</I>
 
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Enter the review inofrmation here.
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<partinfo>BBa_I0500 AddReview 5</partinfo>
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<I>UCL iGEM 2012</I>
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UCL iGEM 2012 have removed the original cobalt inducible promoter to create a stand alone csgBAEFG operon with rbs [https://parts.igem.org/wiki/index.php?title=Part:BBa_K729003 BBa_K729003]. We have also designed two additional variations on the part, with a [https://parts.igem.org/wiki/index.php?title=Part:BBa_K729017 T7] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K729018 constitutive promoter].
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Latest revision as of 19:30, 28 September 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K540000

User Reviews

UNIQ1663b45d3bbdc4db-partinfo-00000000-QINU UNIQ1663b45d3bbdc4db-partinfo-00000001-QINU

•••••

UCL iGEM 2012

UCL iGEM 2012 have removed the original cobalt inducible promoter to create a stand alone csgBAEFG operon with rbs BBa_K729003. We have also designed two additional variations on the part, with a T7 and constitutive promoter.