Difference between revisions of "Part:BBa K777125"
 
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<partinfo>BBa_K777125 short</partinfo>
 
<partinfo>BBa_K777125 short</partinfo>
  
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This part is a combination of a constitutive Anderson promoter with the downstream RFP (for more information check out [https://parts.igem.org/Part:BBa_J61002 J61002] and [https://parts.igem.org/Part:BBa_J23100 J23100]) and the plasmid backbone pSB1C3.  
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[[Image:Table_K777125-K777132.jpg|thumb|300px|right|'''Fig. 1:''' Overview of our related BioBricks with Anderson promoters and RFP in pSB1C3. (Activity according to Berkeley 2006)]]
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This part is a twin of an Anderson promoter in [https://parts.igem.org/Part:BBa_K777133 K777133], a pSB1C3 derived backbone.
  
 
===Usage and Biology===
 
===Usage and Biology===
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To be continued...
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This construct can be useful in several ways, just like the similar part[https://parts.igem.org/Part:BBa_J61002 J61002]. However, if you plan to send coding or composite parts to the registry, they are already in the correct backbone here, [https://parts.igem.org/Part:pSB1C3 pSB1C3].
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# You may use this plasmid to insert a promoter of your choice between the XbaI/EcoRI and SpeI sites. This will provide you with a downstream mRFP as a reporter. Look up the specifications for restriction sites [https://parts.igem.org/Image:PBca1020-r0040.jpg here].
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# Furthermore, you can clone a gene of interest downstream of the constitutive Anderson promoters to express it at a desired rate. For this, the SpeI and PstI sites can be used. Look up the specifications for restriction sites [https://parts.igem.org/Image:PBca1020-r0040.jpg here].
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# It can provide a nice selection system for inserting your basic parts into the pSB1C3 vector via the EcoRI/XbaI and PstI site. If you use a construct containing a strong promoter e.g. K777125, colonies containing a self-ligated plasmid after transformation will turn red and you can select for the non-red colonies.
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<br>
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We also used this construct as a control for our [https://parts.igem.org/Image:YhjH_motility.jpg swimming assays].
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<br><br>
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* Here is the complete sequence as an [https://static.igem.org/mediawiki/2012/0/07/K777125-K777132.zip ApE file]
 
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<partinfo>BBa_K777125 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K777125 SequenceAndFeatures</partinfo>
 
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[[Image:K777125.jpg|frame|left|'''Fig. 1:''' Plasmid map of K777125]]
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[[Image:K777125.jpg|frame|left|'''Fig. 2:''' Plasmid map of K777125]]
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 15:48, 28 September 2012

Constitutive J23100 promoter

Fig. 1: Overview of our related BioBricks with Anderson promoters and RFP in pSB1C3. (Activity according to Berkeley 2006)

This part is a twin of an Anderson promoter in K777133, a pSB1C3 derived backbone.

Usage and Biology

This construct can be useful in several ways, just like the similar partJ61002. However, if you plan to send coding or composite parts to the registry, they are already in the correct backbone here, pSB1C3.

  1. You may use this plasmid to insert a promoter of your choice between the XbaI/EcoRI and SpeI sites. This will provide you with a downstream mRFP as a reporter. Look up the specifications for restriction sites here.
  2. Furthermore, you can clone a gene of interest downstream of the constitutive Anderson promoters to express it at a desired rate. For this, the SpeI and PstI sites can be used. Look up the specifications for restriction sites here.
  3. It can provide a nice selection system for inserting your basic parts into the pSB1C3 vector via the EcoRI/XbaI and PstI site. If you use a construct containing a strong promoter e.g. K777125, colonies containing a self-ligated plasmid after transformation will turn red and you can select for the non-red colonies.


We also used this construct as a control for our swimming assays.

  • Here is the complete sequence as an ApE file



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 8
    Illegal NheI site found at 31
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Fig. 2: Plasmid map of K777125