Difference between revisions of "Part:BBa K771005"
Line 3: | Line 3: | ||
Membrane anchor 5 with light sensor | Membrane anchor 5 with light sensor | ||
+ | Membrane anchor 4, which consists of interacting light sensor VVD ,PDZ ligand and membrane protein Lgt. Membrane Anchor 4 is proved to constitutively dimerize with Membrane Anchor 4 [[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771004 BBa_K771004]]. and 6 [[https://parts.igem.org/wiki/index.php?title=Part:BBa_K771006 BBa_K771006]]. | ||
+ | |||
+ | ==Membrane Accelerator System== | ||
+ | [[Image:Membrane_accelerator.jpg|300px|center]] | ||
+ | |||
+ | |||
+ | All four membrane proteins are assembled together through interacting domains and ligands. Downstream enzymes are thus assembled. | ||
+ | |||
+ | It has been proved to work as expected by recruiting enzymes involved in Fatty Acid biosynthetic pathway. | ||
+ | |||
+ | ==Membrane Rudder System== | ||
+ | [[Image:12SJTU-MA1-MA6.jpg|500px|center]] | ||
+ | Demonstration of the whole ''Membrane Rudder'' device. | ||
===Related Biobrick=== | ===Related Biobrick=== |
Revision as of 13:36, 28 September 2012
Membrane Anchor 5:ssDsbA-VVD-LGT-GBD Ligand
Membrane anchor 5 with light sensor Membrane anchor 4, which consists of interacting light sensor VVD ,PDZ ligand and membrane protein Lgt. Membrane Anchor 4 is proved to constitutively dimerize with Membrane Anchor 4 [BBa_K771004]. and 6 [BBa_K771006].
Membrane Accelerator System
All four membrane proteins are assembled together through interacting domains and ligands. Downstream enzymes are thus assembled.
It has been proved to work as expected by recruiting enzymes involved in Fatty Acid biosynthetic pathway.
Membrane Rudder System
Demonstration of the whole Membrane Rudder device.
Related Biobrick
<br\>Membrabe Anchor 1([BBa_K771001]) <br\>Membrabe Anchor 2([BBa_K771002]) <br\>Membrabe Anchor 3([BBa_K771003]) <br\>Membrabe Anchor 4([BBa_K771004]) <br\>Membrabe Anchor 6([BBa_K771006])
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 494
Illegal AgeI site found at 1339 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 960