Difference between revisions of "Part:BBa K771206"
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Violacein is a violet pigment produced by several bacteria through pathway of 5 related genes, vioA, vioB, vioC, vioD and vioE. It was initially discovered in Chromobacterium violaceum. This metabolite of tryptophan has such special applications as antibacterial, anti-trypanocidal, anti-ulcerogenic, and anticancer drugs. | Violacein is a violet pigment produced by several bacteria through pathway of 5 related genes, vioA, vioB, vioC, vioD and vioE. It was initially discovered in Chromobacterium violaceum. This metabolite of tryptophan has such special applications as antibacterial, anti-trypanocidal, anti-ulcerogenic, and anticancer drugs. | ||
− | + | [[Image:SJTU violacein-pathway.png|Illustration of violacein pathway. The purple part on the left indicates synthetic flux of deoxyviolacein, the green part in the middle indicates synthetic flux of deoxychromoviridans and the violet part on the right indicates synthetic flux of violacein]] | |
The violacein biosynthetic pathway includes 5 key enzymes which work in conjunction. VioA, a flavoenzyme and VioB, a heme protein, work together to oxidize and dimerize tryptophen into IPA imine dimer. Then vioE induces a indole rearrangement,producing prodeoxyviolacein acid, also known as PVA. | The violacein biosynthetic pathway includes 5 key enzymes which work in conjunction. VioA, a flavoenzyme and VioB, a heme protein, work together to oxidize and dimerize tryptophen into IPA imine dimer. Then vioE induces a indole rearrangement,producing prodeoxyviolacein acid, also known as PVA. |
Revision as of 05:29, 28 September 2012
Vio D with Membrane Anchor 6
This part is composed with two parts, the Membrane Anchor 6 and Violacein synthetic pathway enzyme vioD. The enzyme can be aggregated with other membrane anchors, composing the Membrane Magic system. For more information, please visit [http://2012.igem.org/Team:SJTU-BioX-Shanghai 2012 SJTU-BioX-Shanghai wiki]
Violacein biosynthetic pathway
Violacein is a violet pigment produced by several bacteria through pathway of 5 related genes, vioA, vioB, vioC, vioD and vioE. It was initially discovered in Chromobacterium violaceum. This metabolite of tryptophan has such special applications as antibacterial, anti-trypanocidal, anti-ulcerogenic, and anticancer drugs.
The violacein biosynthetic pathway includes 5 key enzymes which work in conjunction. VioA, a flavoenzyme and VioB, a heme protein, work together to oxidize and dimerize tryptophen into IPA imine dimer. Then vioE induces a indole rearrangement,producing prodeoxyviolacein acid, also known as PVA.
VioD that we insert in this part belongs to the last 2 enzymes in this pathway that produce the final main product,violacein.It is a flavin-dependent oxygenase that hydroxylates 5-position indole ring, then the other 2-position indole ring is processed by VioC(see BBa_K771205) to create the oxindole,thus violacein is synthesized.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1465
Illegal XbaI site found at 1477 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1465
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1465
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1465
Illegal XbaI site found at 1477 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1465
Illegal XbaI site found at 1477
Illegal AgeI site found at 494
Illegal AgeI site found at 1339 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 960
Illegal SapI.rc site found at 2458
Illegal SapI.rc site found at 2533