Difference between revisions of "Part:BBa J100099:Experience"
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===Applications of BBa_J100099=== | ===Applications of BBa_J100099=== | ||
| + | We pipetted 200 microliters of one solution containing E coli cells from a small colony and the activators, one with cells from a small colony and no activators, one containing cells from a large colony and the activators, and one containing cells from a large colony and no activators. We also did a positive control with E coli cells containing a known promoter that causes red florescence (J04450) and a negative control with cells containing a the transcriptional terminator that does not cause red fluorescence (J100091). We tested both fluorescence of our samples using a fluorometer and the light absorbance using a spectrophotometer. We measured the fluorescence and absorbance of five samples of each solution, including a control solution that just contained the growth medium. We averaged the values for each solution and subtracted the average fluorescence/absorbance of the control. We then divided the average fluorescence by the average absorbance for each solution. These values are displayed on the accompanying graph. | ||
| + | [[Image:graphs1.png]] | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 19:54, 27 September 2012
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_J100099
We pipetted 200 microliters of one solution containing E coli cells from a small colony and the activators, one with cells from a small colony and no activators, one containing cells from a large colony and the activators, and one containing cells from a large colony and no activators. We also did a positive control with E coli cells containing a known promoter that causes red florescence (J04450) and a negative control with cells containing a the transcriptional terminator that does not cause red fluorescence (J100091). We tested both fluorescence of our samples using a fluorometer and the light absorbance using a spectrophotometer. We measured the fluorescence and absorbance of five samples of each solution, including a control solution that just contained the growth medium. We averaged the values for each solution and subtracted the average fluorescence/absorbance of the control. We then divided the average fluorescence by the average absorbance for each solution. These values are displayed on the accompanying graph.
User Reviews
UNIQ6eaf37a1562d6b84-partinfo-00000000-QINU UNIQ6eaf37a1562d6b84-partinfo-00000001-QINU