Difference between revisions of "Part:BBa K864005"

(New page: __NOTOC__ <partinfo>BBa_K864000 short</partinfo> pSB4A15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance ma...)
 
 
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__NOTOC__
 
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<partinfo>BBa_K864000 short</partinfo>
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<partinfo>BBa_K864005 short</partinfo>
  
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pSB4A15 is a BioBrick standard vector with low copy pSC101 replication origin (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
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pSB4C15(FRT) is a BioBrick standard vector with low copy pSC101 replication origin <partinfo>BBa_K864050</partinfo> (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.
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The resistance cassette is flanked by FRT sites ([[Part:BBa_J61020|BBa_J61020]]) which allows for subsequent removal of the resistance cassette from the chromosome by the FLP recombinase.  
  
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===Usage and Biology===
 
===Usage and Biology===
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This part is available as pSB4A15-[[Part:BBa_K864120|pUCori-red]].  This facilitates high plasmid yields and fast red color expression on transformation plates.
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K864000 parameters</partinfo>
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<partinfo>BBa_K864005 parameters</partinfo>
 
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Latest revision as of 19:19, 27 September 2012

Low copy BioBrick standard vector

pSB4C15(FRT) is a BioBrick standard vector with low copy pSC101 replication origin BBa_K864050 (~5 copies per cell) and ampicillin antibiotic resistance marker, especially usable for Lambda Red recombineering in E coli. The backbone sequence is based on pSB3T5, but the E coli His operon terminator BBa_B0053 has been replaced with the late terminator of the Salmonella phage P22.

The resistance cassette is flanked by FRT sites (BBa_J61020) which allows for subsequent removal of the resistance cassette from the chromosome by the FLP recombinase.

Usage and Biology

This part is available as pSB4A15-pUCori-red. This facilitates high plasmid yields and fast red color expression on transformation plates.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3513
    Illegal NheI site found at 2425
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3519
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3513
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3513
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3513
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3528
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 2319
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.